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Literature DB >> 14425685

Improvement in the histochemical localization of leucine aminopeptidase with a new substrate, L-leucyl-4-methoxy-2-naphthylamide.

M M NACHLAS, B MONIS, D ROSENBATT, A M SELIGMAN.

Abstract

A new method for the histochemical demonstration of leucine aminopeptidase in fresh frozen sections was developed with the substrate L-leucyl-4-methoxy-2-naphthylamide. The superior enzyme localization is due to the more rapid rate of coupling of the hydrolysis product, 4-methoxy-2-naphthylamine as compared to 2-naphthylamine itself, and to the low lipid solubility and high substantivity for protein of the copper chelate of the dye formed on coupling with tetrazotized diorthoanisidine. A comparison of the old and the new method is illustrated, and a description is given of the localization of leucine aminopeptidase in the tissues of the rat and man.

Entities: Chemical Gene Species

Keywords: NAPHTHALENES/chemistry; PROTEASES/chemistry

Mesh：

Substances：

Year: 1960 PMID： 14425685 PMCID： PMC2224813 DOI： 10.1083/jcb.7.2.261

Source DB: PubMed Journal: J Biophys Biochem Cytol ISSN： 0095-9901

11 in total

6. The colorimetric determination of leucine aminopeptidase in urine and serum of normal subjects and patients with cancer and other diseases.

Authors: J A GOLDBARG; A M RUTENBURG
Journal: Cancer Date: 1958 Mar-Apr Impact factor: 6.860

1. Functional polarity of the rat hepatocyte surface membrane. Isolation and characterization of plasma-membrane subfractions from the blood-sinusoidal, bile-Canalicular and contiguous surfaces of the hepatocyte.

Authors: M H Wisher; W H Evans
Journal: Biochem J Date: 1975-02 Impact factor: 3.857

Improvement in the histochemical localization of leucine aminopeptidase with a new substrate, L-leucyl-4-methoxy-2-naphthylamide.

1. Study of leucine aminopeptidase in neoplastic and inflammatory tissues with a new histochemical method.

2. Serum leucine aminopeptidase activity: findings in carcinoma of the pancreas, pregnancy and other disorders.

3. The relationship between fixation and techniques for the histochemical localization of hydrolytic enzymes.

4. Leucine aminopeptidase in pregnancy.

5. The histochemical demonstration of leucine aminopeptidase.

6. The colorimetric determination of leucine aminopeptidase in urine and serum of normal subjects and patients with cancer and other diseases.

7. Histochemical demonstration of aminopeptidase.

8. The colorimetric determination of leucine aminopeptidase activity with L-leucyl-beta-naphthylamide hydrochloride.

9. Chromogenic leucyl substrates for aminopeptidase and papain.

10. The specificity of certain peptidases.

1. Functional polarity of the rat hepatocyte surface membrane. Isolation and characterization of plasma-membrane subfractions from the blood-sinusoidal, bile-Canalicular and contiguous surfaces of the hepatocyte.

2. [ON ENZYME ACTIVITY IN LANGERHANS CELLS].

3. A HISTOCHEMICAL STUDY OF SOME HYDROLYTIC ENZYMES IN TUMOURS OF THE NERVOUS SYSTEM.

4. [THE DEMONSTRATION OF RENAL TUBULAR FUNCTION DISORDERS BY DETERMINATION OF AMINOPEPTIDASE ACTIVITY IN URINE].

5. AMINOPEPTIDASE ACTIVITY IN MYOCARDIAL INFARCTION OF MAN. A HISTOCHEMICAL STUDY.

6. [ON THE ORTHOTOPIC PROPERTY OF HISTOCHEMICALLY DETERMINABLE AMINOPEPTIDASE ACTIVITY OF THE HUMAN SKIN].

7. [STUDIES ON THE CAUSE OF INCREASED LEUCINE AMINOPEPTIDASE ACTIVITY IN THE URINE DURING TUMOR IRRADIATION].

8. [ON THE SO-CALLED LEUCINE AMINOPEPTIDASE OF THE DUODENAL MUCOSA AND THE DUODENAL JUICE].

9. [HISTOCHEMICAL STUDIES OF A GARTNER'S DUCT CARCINOMA OF THE CERVIX UTERI].

10. Morphology and functional cytology of the small intestinal mucosa in malabsorptive disorders and other diseases.