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Literature DB >> 14415404

A diazo coupling method for the electron microscopic localization of cholinesterase.

Abstract

The presence of cholinesterase at the myoneural junction of intercostal muscle has been demonstrated in both light and electron microscopic preparations. A new simultaneous diazo coupling technique using alpha-naphthyl acetate as substrate and "hexazonium pararosanilin" as coupler has been applied to cold formalin-fixed tissues. After postfixation in buffered osmium tetroxide the sites of esterase activity are faithfully demonstrated at a high level of resolution. The details of cholin-esterase distribution and some technical aspects of the procedure are discussed.

Entities: Chemical Gene

Keywords: CHOLINESTERASE/chemistry

Mesh：

Substances：

Year: 1959 PMID： 14415404 PMCID： PMC2224705 DOI： 10.1083/jcb.6.3.399

Source DB: PubMed Journal: J Biophys Biochem Cytol ISSN： 0095-9901

17 in total

1. The relationship between fixation and techniques for the histochemical localization of hydrolytic enzymes.

Authors: M S BURSTONE
Journal: J Histochem Cytochem Date: 1958-09 Impact factor: 2.479

2. Histochemical reactions for electron microscopy: acid phosphatase.

Authors: H SHELDON; H ZETTERQVIST; D BRANDES
Journal: Exp Cell Res Date: 1955-12 Impact factor: 3.905

3. Studies in enzyme cytochemistry I. Principles of cytochemical staining methods.

Authors: S J HOLT; D G O'SULLIVAN
Journal: Proc R Soc Lond B Biol Sci Date: 1958-04-08

4. Applications of histochemistry to electron microscopy.

Authors: R J BARRNETT; G E PALADE
Journal: J Histochem Cytochem Date: 1958-01 Impact factor: 2.479

5. Histochemical techniques for electron microscopy: alkaline phosphatase.

Authors: D BRANDES; H ZETTERQVIST; H SHELDON
Journal: Nature Date: 1956-02-25 Impact factor: 49.962

6. On the histochemistry of the myoneural junction.

Authors: F A DENZ
Journal: Br J Exp Pathol Date: 1953-06

7. [Effect of formaldehyde on activity of various preparations of cholinesterase].

Authors: J TAXI
Journal: J Physiol (Paris) Date: 1952

8. The elimination of enzymatic diffusion artifacts in the histochemical localization of cholinesterases and a survey of their cellular distributions.

Authors: G B KOELLE
Journal: J Pharmacol Exp Ther Date: 1951-10 Impact factor: 4.030

9. "Osmiophilia, "fact or fiction.

Authors: L ORNSTEIN
Journal: J Biophys Biochem Cytol Date: 1957-09-25

10. Adenosinetriphosphatase and 5-nucleotidase activities in the plasma membrane of liver cells as revealed by electron microscopy.

Authors: E ESSNER; A B NOVIKOFF; B MASEK
Journal: J Biophys Biochem Cytol Date: 1958-11-25

50 in total

1. PARTIAL PURIFICATION OF SOLUBLE RNA.

Authors: P C Zamecnik; M L Stephenson; J F Scott
Journal: Proc Natl Acad Sci U S A Date: 1960-06 Impact factor: 11.205

2. Simultaneous localization of acetylcholinesterase activity and met-enkephalin, vasoactive intestinal peptide and substance P immunoreactivity in the rat myenteric plexus.

Authors: P Anglade; C Michel; T Ozaki; S Tsuji; X Vignon; N Yanaihara
Journal: Histochem J Date: 1988-08

7. Electron-cytochemical demonstration of acid phosphatase and thiolacetic acid-esterase activity in granular structures of the phagotrophic slime mold, Physarum confertum Macbr.

Authors: L Bauer
Journal: Histochemie Date: 1973

A diazo coupling method for the electron microscopic localization of cholinesterase.

1. The relationship between fixation and techniques for the histochemical localization of hydrolytic enzymes.

2. Histochemical reactions for electron microscopy: acid phosphatase.

3. Studies in enzyme cytochemistry I. Principles of cytochemical staining methods.

4. Applications of histochemistry to electron microscopy.

5. Histochemical techniques for electron microscopy: alkaline phosphatase.

6. On the histochemistry of the myoneural junction.

7. [Effect of formaldehyde on activity of various preparations of cholinesterase].

8. The elimination of enzymatic diffusion artifacts in the histochemical localization of cholinesterases and a survey of their cellular distributions.

9. "Osmiophilia, "fact or fiction.

10. Adenosinetriphosphatase and 5-nucleotidase activities in the plasma membrane of liver cells as revealed by electron microscopy.

1. PARTIAL PURIFICATION OF SOLUBLE RNA.

2. Simultaneous localization of acetylcholinesterase activity and met-enkephalin, vasoactive intestinal peptide and substance P immunoreactivity in the rat myenteric plexus.

3. Postnatal maturation of spindles in deafferented rat soleus muscles.

4. Esterase. II. A new method for the electron microscopical demonstration of a nonspecific esterase in animal tissues.

5. Localization of acetylcholinesterase in the rat myoneural junction.

6. Fluorescent staining of acetylcholine receptors in vertebrate skeletal muscle.

7. Electron-cytochemical demonstration of acid phosphatase and thiolacetic acid-esterase activity in granular structures of the phagotrophic slime mold, Physarum confertum Macbr.

8. Neurotrophic regulation of muscle cholinesterase: effects of botulinum toxin and denervation.

9. Histochemical profiles of cat intrafusal muscle fibers and their motor innervation.

10. Cell specialization in the small intestinal epithelium of adult Xenopus laevis: functional aspects.