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Literature DB >> 1438220

DnaJ, DnaK, and GrpE heat shock proteins are required in oriP1 DNA replication solely at the RepA monomerization step.

S Wickner¹, D Skowyra, J Hoskins, K McKenney.

Abstract

We have found that three Escherichia coli heat shock proteins, DnaK (the hsp70 homolog), DnaJ, and GrpE, function in oriP1 DNA replication in vitro solely to activate DNA binding by the replication initiator protein RepA. Activation results from the conversion of P1 or P7 RepA dimers to monomers that bind with high affinity to the origin of replication of plasmid P1. Thus, the essential role of these three heat shock proteins in this replication system is to change the quaternary structure of a single protein, RepA.

Entities: Disease Gene Species

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Year: 1992 PMID： 1438220 PMCID： PMC50335 DOI： 10.1073/pnas.89.21.10345

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

27 in total

1. Function of DnaJ and DnaK as chaperones in origin-specific DNA binding by RepA.

Authors: S Wickner; J Hoskins; K McKenney
Journal: Nature Date: 1991-03-14 Impact factor: 49.962

Review 2. Protein folding in the cell.

Authors: M J Gething; J Sambrook
Journal: Nature Date: 1992-01-02 Impact factor: 49.962

3. Monomerization of RepA dimers by heat shock proteins activates binding to DNA replication origin.

Authors: S Wickner; J Hoskins; K McKenney
Journal: Proc Natl Acad Sci U S A Date: 1991-09-15 Impact factor: 11.205

Review 4. Polypeptide chain binding proteins: catalysts of protein folding and related processes in cells.

Authors: J E Rothman
Journal: Cell Date: 1989-11-17 Impact factor: 41.582

5. A single amino acid difference between Rep proteins of P1 and P7 affects plasmid copy number.

Authors: B J Froehlich; J R Scott
Journal: Plasmid Date: 1988-03 Impact factor: 3.466

6. The grpE protein of Escherichia coli. Purification and properties.

Authors: M Zylicz; D Ang; C Georgopoulos
Journal: J Biol Chem Date: 1987-12-25 Impact factor: 5.157

7. Specialized nucleoprotein structures at the origin of replication of bacteriophage lambda: localized unwinding of duplex DNA by a six-protein reaction.

Authors: M Dodson; H Echols; S Wickner; C Alfano; K Mensa-Wilmot; B Gomes; J LeBowitz; J D Roberts; R McMacken
Journal: Proc Natl Acad Sci U S A Date: 1986-10 Impact factor: 11.205

8. Speculations on the functions of the major heat shock and glucose-regulated proteins.

Authors: H R Pelham
Journal: Cell Date: 1986-09-26 Impact factor: 41.582

9. Deletion analysis of the mini-P1 plasmid origin of replication and the role of Escherichia coli DnaA protein.

Authors: S Wickner; J Hoskins; D Chattoraj; K McKenney
Journal: J Biol Chem Date: 1990-07-15 Impact factor: 5.157

10. Purified dnaA protein in initiation of replication at the Escherichia coli chromosomal origin of replication.

Authors: R S Fuller; A Kornberg
Journal: Proc Natl Acad Sci U S A Date: 1983-10 Impact factor: 11.205

26 in total

1. Dimers of pi protein bind the A+T-rich region of the R6K gamma origin near the leading-strand synthesis start sites: regulatory implications.

Authors: R Krüger; M Filutowicz
Journal: J Bacteriol Date: 2000-05 Impact factor: 3.490

10. Replication initiator protein RepE of mini-F plasmid: functional differentiation between monomers (initiator) and dimers (autogenous repressor).

Authors: M Ishiai; C Wada; Y Kawasaki; T Yura
Journal: Proc Natl Acad Sci U S A Date: 1994-04-26 Impact factor: 11.205

DnaJ, DnaK, and GrpE heat shock proteins are required in oriP1 DNA replication solely at the RepA monomerization step.

1. Function of DnaJ and DnaK as chaperones in origin-specific DNA binding by RepA.

Review 2. Protein folding in the cell.

3. Monomerization of RepA dimers by heat shock proteins activates binding to DNA replication origin.

Review 4. Polypeptide chain binding proteins: catalysts of protein folding and related processes in cells.

5. A single amino acid difference between Rep proteins of P1 and P7 affects plasmid copy number.

6. The grpE protein of Escherichia coli. Purification and properties.

7. Specialized nucleoprotein structures at the origin of replication of bacteriophage lambda: localized unwinding of duplex DNA by a six-protein reaction.

8. Speculations on the functions of the major heat shock and glucose-regulated proteins.

9. Deletion analysis of the mini-P1 plasmid origin of replication and the role of Escherichia coli DnaA protein.

10. Purified dnaA protein in initiation of replication at the Escherichia coli chromosomal origin of replication.

1. Dimers of pi protein bind the A+T-rich region of the R6K gamma origin near the leading-strand synthesis start sites: regulatory implications.

2. The DnaK chaperone is necessary for alpha-complementation of beta-galactosidase in Escherichia coli.

Review 3. Remodeling protein complexes: insights from the AAA+ unfoldase ClpX and Mu transposase.

4. Protein domains and conformational changes in the activation of RepA, a DNA replication initiator.

5. Mechanisms of Theta Plasmid Replication.

6. Involvement of the DnaK-DnaJ-GrpE chaperone team in protein secretion in Escherichia coli.

7. The DnaJ domain of polyomavirus large T antigen is required to regulate Rb family tumor suppressor function.

8. Interaction of murine BiP/GRP78 with the DnaJ homologue MTJ1.

9. HSF1 overexpression enhances oncolytic effect of replicative adenovirus.

10. Replication initiator protein RepE of mini-F plasmid: functional differentiation between monomers (initiator) and dimers (autogenous repressor).