Literature DB >> 1438216

Somatic recombination of heavy chain variable region transgenes with the endogenous immunoglobulin heavy chain locus in mice.

A M Giusti1, R Coffee, T Manser.   

Abstract

Transgenic lines of mice were derived by using plasmid constructs containing DNA encoding an antibody heavy chain variable-diversity-joining region (VH-D-JH) and various amounts of 5' and 3' flanking DNA but lacking any repetitive isotype switch (S) or constant (C) region DNA. Unexpectedly, many of the antibody VH regions expressed by B-cell hybridomas generated from immunized transgenic mice were found to be of transgenic origin. Further analyses showed that somatic events had generated hybrid genomic loci in the mice containing the transgenic VH-D-JH gene and plasmid sequences 5' of endogenous heavy chain C region genes. Thus, VH-D-JH transgenes lacking S and C region DNA can recombine with endogenous Igh DNA, leading to the expression of transgene-encoded antibody.

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Year:  1992        PMID： 1438216      PMCID： PMC50330          DOI： 10.1073/pnas.89.21.10321

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  25 in total

1.  Molecular mapping of idiotopes of anti-arsonate antibodies.

Authors:  D Jeske; E C Milner; O Leo; M Moser; J Marvel; J Urbain; J D Capra
Journal:  J Immunol       Date:  1986-04-01       Impact factor: 5.422

2.  Mitogen-driven B cell proliferation and differentiation are not accompanied by hypermutation of immunoglobulin variable region genes.

Authors:  T Manser
Journal:  J Immunol       Date:  1987-07-01       Impact factor: 5.422

3.  A novel VH to VHDJH joining mechanism in heavy-chain-negative (null) pre-B cells results in heavy-chain production.

Authors:  M Reth; P Gehrmann; E Petrac; P Wiese
Journal:  Nature       Date:  1986 Aug 28-Sep 3       Impact factor: 49.962

Review 4.  Immunoglobulin gene transcription: molecular mechanisms.

Authors:  K L Calame
Journal:  Trends Genet       Date:  1989-12       Impact factor: 11.639

5.  Altered repertoire of endogenous immunoglobulin gene expression in transgenic mice containing a rearranged mu heavy chain gene.

Authors:  D Weaver; M H Reis; C Albanese; F Costantini; D Baltimore; T Imanishi-Kari
Journal:  Cell       Date:  1986-04-25       Impact factor: 41.582

Review 6.  Evolution of antibody variable region structure during the immune response.

Authors:  T Manser; L J Wysocki; M N Margolies; M L Gefter
Journal:  Immunol Rev       Date:  1987-04       Impact factor: 12.988

Review 7.  Somatic generation of antibody diversity.

Authors:  S Tonegawa
Journal:  Nature       Date:  1983-04-14       Impact factor: 49.962

8.  A mouse immunoglobulin heavy chain deletion mutant: isolation of a cDNA clone and sequence analysis of the mRNA.

Authors:  W Dunnick; T H Rabbitts; C Milstein
Journal:  Nucleic Acids Res       Date:  1980-04-11       Impact factor: 16.971

9.  Isotype switching of an immunoglobulin heavy chain transgene occurs by DNA recombination between different chromosomes.

Authors:  R M Gerstein; W N Frankel; C L Hsieh; J M Durdik; S Rath; J M Coffin; A Nisonoff; E Selsing
Journal:  Cell       Date:  1990-11-02       Impact factor: 41.582

10.  Evolution of antibody structure during the immune response. The differentiative potential of a single B lymphocyte.

Authors:  T Manser
Journal:  J Exp Med       Date:  1989-10-01       Impact factor: 14.307
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  10 in total

1.  Trans-chromosomal recombination within the Ig heavy chain switch region in B lymphocytes.

Authors:  M Kingzette; H Spieker-Polet; P C Yam; S K Zhai; K L Knight
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-29       Impact factor: 11.205

2.  Enhancement of antibody class-switch recombination by the cumulative activity of four separate elements.

Authors:  Wesley A Dunnick; Jian Shi; Jennifer M Zerbato; Clinton A Fontaine; John T Collins
Journal:  J Immunol       Date:  2011-09-26       Impact factor: 5.422

3.  Ectopic expression of A-myb in transgenic mice causes follicular hyperplasia and enhanced B lymphocyte proliferation.

Authors:  S E DeRocco; R Iozzo; X P Ma; R Schwarting; D Peterson; B Calabretta
Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-01       Impact factor: 11.205

4.  Translocation of an antibody transgene requires AID and occurs by interchromosomal switching to all switch regions except the mu switch region.

Authors:  Maryam Shansab; Jennifer M Eccleston; Erik Selsing
Journal:  Eur J Immunol       Date:  2011-04-11       Impact factor: 5.532

5.  The transcriptional promoter regulates hypermutation of the antibody heavy chain locus.

Authors:  K Tumas-Brundage; T Manser
Journal:  J Exp Med       Date:  1997-01-20       Impact factor: 14.307

6.  The promotion of V region hypermutation.

Authors:  M D Scharff; V Poltoratsky; N S Green
Journal:  J Exp Med       Date:  1997-01-20       Impact factor: 14.307

7.  Spontaneous autoimmunity in mice that carry an IghV partial transgene: a required arginine in VHCDR3.

Authors:  A Guth; T Detanico; D Smith; K S K Tung; C Bonorino; L J Wysocki
Journal:  Lupus       Date:  2009-04       Impact factor: 2.911

8.  Somatic generation of hybrid antibody H chain genes in transgenic mice via interchromosomal gene conversion.

Authors:  A M Giusti; T Manser
Journal:  J Exp Med       Date:  1994-01-01       Impact factor: 14.307

9.  Altering the antibody repertoire via transgene homologous recombination: evidence for global and clone-autonomous regulation of antigen-driven B cell differentiation.

Authors:  K A Vora; T Manser
Journal:  J Exp Med       Date:  1995-01-01       Impact factor: 14.307

10.  Hypermutation is observed only in antibody H chain V region transgenes that have recombined with endogenous immunoglobulin H DNA: implications for the location of cis-acting elements required for somatic mutation.

Authors:  A M Giusti; T Manser
Journal:  J Exp Med       Date:  1993-03-01       Impact factor: 14.307
  10 in total

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