Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 THE FLUORIMETRIC MICRODETERMINATION OF GLYOXYLIC ACID IN BLOOD, URINE AND BACTERIAL EXTRACTS.

Literature DB >> 14343135

THE FLUORIMETRIC MICRODETERMINATION OF GLYOXYLIC ACID IN BLOOD, URINE AND BACTERIAL EXTRACTS.

Abstract

1. A spectrophotofluorimetric method for the determination of glyoxylic acid in biological materials is described. 2. The method is based on the reaction between glyoxylic acid and resorcinol in acid solution, a fluorescent complex being obtained on the subsequent addition of alkali. 3. The reaction was found to be sensitive and highly specific, the minimum detectable amount of glyoxylic acid being 1.35x10(-8) mole. 4. The urinary excretion of glyoxylic acid by ten normal adults ranged from 1.4 to 4.7mg./24hr. Small but measurable amounts of glyoxylic acid were found in cell-free extracts of Pseudomonas oxalaticus OX1 grown on oxalic acid as a source of carbon. No glyoxylic acid was detected in human serum.

Entities: Chemical Species

Keywords: ACETATES; BLOOD CHEMICAL ANALYSIS; EXPERIMENTAL LAB STUDY; FLUORESCENCE; FLUOROMETRY; PSEUDOMONAS; RESORCINOL; SPECTRUM ANALYSIS; URINE

Mesh：

Substances：

Year: 1965 PMID： 14343135 PMCID： PMC1206925 DOI： 10.1042/bj0960218

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

14 in total

Review 1. PRIMARY HYPEROXALURIA.

Authors: T D HOCKADAY; J E CLAYTON; E W FREDERICK; L H SMITH
Journal: Medicine (Baltimore) Date: 1964-05 Impact factor: 1.889

2. THE GLYOXYLIC ACID CONTENT OF HUMAN BLOOD AND ITS RELATIONSHIP TO THIAMINE DEFICIENCY.

Authors: R M BUCKLE
Journal: Clin Sci Date: 1963-10 Impact factor: 6.124

3. Conversion of gamma-hydroxyglutamate to glyoxylate and alanine; purification and properties of the enzyme system.

Authors: E E DEKKER; U MAITRA
Journal: J Biol Chem Date: 1962-07 Impact factor: 5.157

4. [On the technic for paper chromatographic separation and determination of alpha-keto acids in blood].

Authors: H KAESER
Journal: Clin Chim Acta Date: 1961-05 Impact factor: 3.786

5. Spectrophotometric measurement of alpha-keto acid semicarbazones.

Authors: J A OLSON
Journal: Arch Biochem Biophys Date: 1959-11 Impact factor: 4.013

6. [Blood and urinary keto acids: analytic study; identification of new hydrazones; clinical applications].

Authors: G BISERTE; B DASSONVILLE
Journal: Clin Chim Acta Date: 1956-01 Impact factor: 3.786

7. The structure of ribonucleic acids. II. The smaller products of ribonuclease digestion.

Authors: R MARKHAM; J D SMITH
Journal: Biochem J Date: 1952-12 Impact factor: 3.857

8. Carbon assimilation by Pseudomonas oxalaticus (OXI). 4. Metabolism of oxalate in cell-free extracts of the organism grown on oxalate.

Authors: J R Quayle; D B Keech; G A Taylor
Journal: Biochem J Date: 1961-02 Impact factor: 3.857

THE FLUORIMETRIC MICRODETERMINATION OF GLYOXYLIC ACID IN BLOOD, URINE AND BACTERIAL EXTRACTS.

Review 1. PRIMARY HYPEROXALURIA.

2. THE GLYOXYLIC ACID CONTENT OF HUMAN BLOOD AND ITS RELATIONSHIP TO THIAMINE DEFICIENCY.

3. Conversion of gamma-hydroxyglutamate to glyoxylate and alanine; purification and properties of the enzyme system.

4. [On the technic for paper chromatographic separation and determination of alpha-keto acids in blood].

5. Spectrophotometric measurement of alpha-keto acid semicarbazones.

6. [Blood and urinary keto acids: analytic study; identification of new hydrazones; clinical applications].

7. The structure of ribonucleic acids. II. The smaller products of ribonuclease digestion.

8. Carbon assimilation by Pseudomonas oxalaticus (OXI). 4. Metabolism of oxalate in cell-free extracts of the organism grown on oxalate.

9. Studies on experimental thiamine deficiency. Trends of keto acid formtion and detection of glyoxylic acid.

10. Studies in dialysis; changes of pH during dialysis.

1. Analytical isotachophoresis: a rapid and sensitive method for determination of urinary oxalate.

2. Calcium phosphate and oxalate ion-products in normal and stone-forming urines.

3. The fluorimetric determination of oxalic acid in blood and other biological materials.

4. Oxidation of pyrimidine nucleosides and nucleotides by osmium tetroxide.

5. Reference values of plasma oxalate in children and adolescents.