Literature DB >> 14335815

THE SEPARATION OF FERRITIN AND HAEMOSIDERIN FOR STUDIES IN THE METABOLISM OF IRON.

J W DRYSDALE, W N RAMSAY.   

Abstract

1. Ferritin and haemosiderin in diluted tissue cannot be quantitatively separated by centrifugation unless the pH is brought below 6. 2. These iron-storage substances can be separated from one another and from haemoglobin by ion-exchange chromatography on CM-cellulose, when good recoveries of total tissue iron are obtained. 3. The ferritin fraction has been identified by its solubility, its appearance under the electron microscope, crystallization with Cd(2+) and the fact that it corresponds quantitatively to the heat-stable portion of the tissue non-haem iron.

Entities:  

Keywords:  BIOCHEMISTRY; CADMIUM; CENTRIFUGATION; CHROMATOGRAPHY; CRYSTALLIZATION; EXPERIMENTAL LAB STUDY; FERRITIN; HEAT; HEMOSIDERIN; HOMOGENATES; HYDROGEN-ION CONCENTRATION; ION EXCHANGE RESINS; IRON METABOLISM; LIVER; RATS; SOLUBILITY; SPLEEN

Mesh：

Substances：

Year:  1965        PMID： 14335815      PMCID： PMC1215205          DOI： 10.1042/bj0950282

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  17 in total

1.  Hemosiderin; isolation from horse spleen and characterization.

Authors:  S LUDEWIG
Journal:  Proc Soc Exp Biol Med       Date:  1957-07

2.  Studies on intermediary iron metabolism. XII. Measurement of the iron derived from water soluble and water insoluble non-haem compounds (ferritin and haemosiderin iron) in liver and spleen.

Authors:  I KALDOR
Journal:  Aust J Exp Biol Med Sci       Date:  1958-04

3.  The effects of acute protein depletion on the distribution of radioiron in rat tissues.

Authors:  W F BETHARD; R W WISSLER; J S THOMPSON; M A SCHROEDER; M J ROBSON
Journal:  Blood       Date:  1958-02       Impact factor: 22.113

4.  Iron metabolism in the laying hen.

Authors:  W N RAMSAY; E A CAMPBELL
Journal:  Biochem J       Date:  1954-10       Impact factor: 3.857

5.  [Physiology and pathology of stored iron. 1. A method for quantitative determination of ferritin].

Authors:  W KEIDERLING; F WOHLER
Journal:  Naunyn Schmiedebergs Arch Exp Pathol Pharmakol       Date:  1954

6.  An electron microscopic study of ferritin.

Authors:  J L FARRANT
Journal:  Biochim Biophys Acta       Date:  1954-04

7.  Quantitative fractionation of tissue ferritin and hemosiderin.

Authors:  B W GABRIO; A SHODEN; C A FINCH
Journal:  J Biol Chem       Date:  1953-10       Impact factor: 5.157

8.  The relationship between ferritin and hemosiderin in rabbits and man.

Authors:  A SHODEN; B W GABRIO; C A FINCH
Journal:  J Biol Chem       Date:  1953-10       Impact factor: 5.157

9.  Uptake and storage of radioactive iron by mouse liver.

Authors:  J K HAMPTON; J B KAHN
Journal:  Am J Physiol       Date:  1953-08

10.  A study of hemosiderosis with the aid of electron microscopy; with observations on the relationship between hemosiderin and ferritin.

Authors:  G W RICHTER
Journal:  J Exp Med       Date:  1957-08-01       Impact factor: 14.307
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  9 in total

1.  Determination of nonheme iron using inductively coupled plasma-atomic emission spectrometry.

Authors:  K Yokoi; M Kimura; Y Itokawa
Journal:  Biol Trace Elem Res       Date:  1991-12       Impact factor: 3.738

2.  Iron accumulation in tissues of magnesium-deficient rats with dietary iron overload.

Authors:  M Kimura; K Yokoi
Journal:  Biol Trace Elem Res       Date:  1996-02       Impact factor: 3.738

3.  Hepatic iron deprivation prevents spontaneous development of fulminant hepatitis and liver cancer in Long-Evans Cinnamon rats.

Authors:  J Kato; M Kobune; Y Kohgo; N Sugawara; H Hisai; T Nakamura; S Sakamaki; N Sawada; Y Niitsu
Journal:  J Clin Invest       Date:  1996-08-15       Impact factor: 14.808

4.  Heterogeneity in tissue ferritins displayed by gel electrofocusing.

Authors:  J W Drysdale
Journal:  Biochem J       Date:  1974-09       Impact factor: 3.857

5.  Denatured H-ferritin subunit is a major constituent of haemosiderin in the liver of patients with iron overload.

Authors:  E Miyazaki; J Kato; M Kobune; K Okumura; K Sasaki; N Shintani; P Arosio; Y Niitsu
Journal:  Gut       Date:  2002-03       Impact factor: 23.059

6.  SMALL-SCALE ISOLATION OF FERRITIN FOR THE ASSAY OF THE INCORPORATION OF 14C-LABELLED AMINO ACIDS.

Authors:  J W DRYSDALE; H N MUNRO
Journal:  Biochem J       Date:  1965-06       Impact factor: 3.857

7.  Effect of actinomycin D, cycloheximide, and acute blood loss of ferritin synthesis in rat liver.

Authors:  J A Millar; R L Cumming; J A Smith; A Goldberg
Journal:  Biochem J       Date:  1970-10       Impact factor: 3.857

8.  Isolation and characterization of Phycomyces blakesleeanus ferritin.

Authors:  V J LaBombardi; M A Pisano; J V Klavins
Journal:  J Bacteriol       Date:  1982-05       Impact factor: 3.490

9.  Modulation of exudate inflammation parameters in rat carrageenan-induced granuloma by modification of exudate iron levels.

Authors:  J Muntane; P Fritsch; T Carbonell; M P Saiz; P Puig-Parellada; M T Mitjavila
Journal:  Agents Actions       Date:  1991-03
  9 in total

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