Comparison of the properties of the ATP-sensitive K+ channels of pancreatic beta-cells of lean and obese (ob/ob) C57BL/6J mice.

Cultures of pancreatic islet cells from obese and lean mice of the C57BL/6J strain were established and their secretory response to glucose stimulation was measured. Insulin secretion (as % of total cellular insulin content) from the cells of the obese mouse cultures was significantly higher than from lean mouse cells. The properties of the glucose- and ATP-sensitive potassium channels present in these cultured beta-cells were compared using the cell-attached and the inside-out configurations of the patch-clamp technique. The channels of both types of mouse were indistinguishable in terms of conductance, ionic selectivity, kinetic behavior, voltage dependence or sensitivity to glucose, ATP and ADP. It is concluded that the depolarized state and the hypersecretory response of obese mouse beta-cells are not related to an altered behavior of their ATP-sensitive potassium channels.