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Literature DB >> 14326114

LOCALIZATION OF THE SUCCINIC DEHYDROGENASE SYSTEM IN ESCHERICHIA COLI USING COMBINED TECHNIQUES OF CYTOCHEMISTRY AND ELECTRON MICROSCOPY.

Abstract

The activity of the succinic dehydrogenase system was studied in Escherichia coli utilizing combined techniques of cytochemistry and electron microscopy. Organisms were incubated in a medium containing tetranitro-blue tetrazolium (TNBT) which served as an electron acceptor. Enzymatic activity, as evidenced by the deposition of aggregates of TNBT-formazan, was found associated with the site of the plasma membrane of the bacterium.

Entities: Chemical Disease Species

Keywords: CHEMISTRY; CULTURE MEDIA; ESCHERICHIA COLI; EXPERIMENTAL LAB STUDY; MICROSCOPY, ELECTRON; SUCCINATE DEHYDROGENASE; TETRAZOLIUM SALTS

Mesh：

Substances：

Year: 1965 PMID： 14326114 PMCID： PMC2106567 DOI： 10.1083/jcb.24.2.285

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

12 in total

1. High resolution electron microscopy on the surface structure of Escherichia coli.

Authors: M OGURA
Journal: J Ultrastruct Res Date: 1963-04

2. [Bacterial intracytoplasmic organelles and the site of oxidation-reduction activity].

Authors: E VANDERWINKEL; R G MURRAY
Journal: J Ultrastruct Res Date: 1962-08

3. The fine structure of bacteria.

Authors: A M GLAUERT
Journal: Br Med Bull Date: 1962-09 Impact factor: 4.291

4. Light and electron microscopic studies of Escherichia coli-coliphage interactions. III. Persistence of mitochrondria and reductase activity during infection of Escherichia coli B with T2 phage.

Authors: P E HARTMAN; S MUDD; J HILLIER; E H BEUTNER
Journal: J Bacteriol Date: 1953-06 Impact factor: 3.490

5. Use of tetrazolium compounds in oxidative enzyme histo- and cytochemistry.

Authors: C G ROSA; K C TSOU
Journal: Nature Date: 1961-12-09 Impact factor: 49.962

6. Further evidence of the existence of mitochondria in bacteria.

Authors: S MUDD; A F BRODIE; L C WINTERSCHEID; P E HARTMAN; E H BEUTNER; R A McLEAN
Journal: J Bacteriol Date: 1951-12 Impact factor: 3.490

7. Improvements in epoxy resin embedding methods.

Authors: J H LUFT
Journal: J Biophys Biochem Cytol Date: 1961-02

8. Cell wall and cytoplasmic membrane of Escherichia coli.

Authors: E KELLENBERGER; A RYTER
Journal: J Biophys Biochem Cytol Date: 1958-05-25

9. A CYTOCHEMICAL LOCALIZATION OF REDUCTIVE SITES IN A GRAM-NEGATIVE BACTERIUM. TELLURITE REDUCTION IN PROTEUS VULGARIS.

Authors: W LEENE
Journal: J Cell Biol Date: 1964-03 Impact factor: 10.539

10. The use of tetranitro-blue tetrazolium for the cytochemical localization of succinic dehydrogenase. Cytochemical and cytological studies of sarcoma 37 ascites tumor cells.

Authors: C G ROSA; K C TSOU
Journal: J Cell Biol Date: 1963-03 Impact factor: 10.539

9 in total

8. The demonstration of the succinic dehydrogenase system in Bacillus subtilis using tetranitro--blue tetrazolium combined with techniques of electron microscopy.

Authors: A W Sedar; R M Burde
Journal: J Cell Biol Date: 1965-10 Impact factor: 10.539

9. Tetranitro--blue tetrazolium reduction in Proteus vulgaris.

Authors: W Leene; W van Iterson
Journal: J Cell Biol Date: 1965-10 Impact factor: 10.539

9 in total

LOCALIZATION OF THE SUCCINIC DEHYDROGENASE SYSTEM IN ESCHERICHIA COLI USING COMBINED TECHNIQUES OF CYTOCHEMISTRY AND ELECTRON MICROSCOPY.

1. High resolution electron microscopy on the surface structure of Escherichia coli.

2. [Bacterial intracytoplasmic organelles and the site of oxidation-reduction activity].

3. The fine structure of bacteria.

4. Light and electron microscopic studies of Escherichia coli-coliphage interactions. III. Persistence of mitochrondria and reductase activity during infection of Escherichia coli B with T2 phage.

5. Use of tetrazolium compounds in oxidative enzyme histo- and cytochemistry.

6. Further evidence of the existence of mitochondria in bacteria.

7. Improvements in epoxy resin embedding methods.

8. Cell wall and cytoplasmic membrane of Escherichia coli.

9. A CYTOCHEMICAL LOCALIZATION OF REDUCTIVE SITES IN A GRAM-NEGATIVE BACTERIUM. TELLURITE REDUCTION IN PROTEUS VULGARIS.

10. The use of tetranitro-blue tetrazolium for the cytochemical localization of succinic dehydrogenase. Cytochemical and cytological studies of sarcoma 37 ascites tumor cells.

1. Histochemical localization of phosphatases in Mycoplasma gallisepticum.

2. [Localization of reductive sites in bacteria by methylenblue-silver (Argochrome) with the electron microscope].

3. ACRIFLAVINE INDUCTION OF DIFFERENT KINDS OF "PETITE" MITOCHONDRIAL POPULATIONS IN SACCHAROMYCES CEREVISIAE.

4. Effects of oleate starvation in a fatty acid auxotroph of Escherichia coli K-12.

5. Limiting availability of binding sites for dehydrogenases on the cell membrane of Escherichia coli.

6. Respiration-driven proton translocation in Escherichia coli.

7. The formation of multiple layers of membrane-like structures in Escherichia coli B.

8. The demonstration of the succinic dehydrogenase system in Bacillus subtilis using tetranitro--blue tetrazolium combined with techniques of electron microscopy.

9. Tetranitro--blue tetrazolium reduction in Proteus vulgaris.