| Literature DB >> 14325889 |
Abstract
A cooling cell is described that facilitates maintenance of biological materials at low temperatures during prolonged sonic treatment. Using this cell and a Branson S-75 Sonifier, I examined temperatures and the release of protein and enzyme activity from suspensions of Saccharomyces cerevisiae. With the Sonifier at full power, it was possible to maintain cell temperature within 9 C of the cooling-bath temperature, and to disrupt 10% (w/v) suspensions of S. cerevisiae in 10 min.Entities:
Keywords: BACTERIOLOGICAL TECHNICS; COLD; EQUIPMENT AND SUPPLIES; EXPERIMENTAL LAB STUDY; OSCILLOMETRY; SACCHAROMYCES; TOXICOLOGIC REPORT
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Year: 1965 PMID： 14325889 PMCID： PMC1058231 DOI： 10.1128/am.13.2.254-256.1965
Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919