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Literature DB >> 14325469

FORMATION OF CHARCOT-LEYDEN CRYSTALS IN HUMAN EOSINOPHILS AND BASOPHILS AND STUDY OF THE COMPOSITION OF ISOLATED CRYSTALS.

Abstract

Methods are described for the isolation of human eosinophils and basophils from donor blood. Using these cell preparations Charcot-Leyden crystals were found to originate from both eosinophils and basophils when the cells were suspended in hypotonic saline solution. The crystals formed also when saline extracts of eosinophils and basophils were concentrated by ultrafiltration through dialysis tubing. Fractions of eosinophils were prepared and the crystals were obtained from the cytoplasmic fraction but not from the nuclear or granular fractions. Chemical studies showed the crystals to be protein in nature and some evidence is presented which suggests that RNA may decrease the tendency for the protein to crystallize out of solution.

Entities: Chemical Disease Species

Keywords: BASOPHILS; BLOOD CHEMICAL ANALYSIS; CHROMATOGRAPHY; EOSINOPHILIA; EOSINOPHILS; MICROSCOPY, PHASE CONTRAST; PATHOLOGY; PROTEINS; RNA

Mesh：

Substances：

Year: 1965 PMID： 14325469 PMCID： PMC2138026 DOI： 10.1084/jem.122.1.173

Source DB: PubMed Journal: J Exp Med ISSN： 0022-1007 Impact factor: 14.307

8 in total

5. [Demonstration by electrophoresis of the relation between ribonucleic acid, non-centrifugable at high speed, and cytoplasmic proteins].

Authors: D SZAFARZ
Journal: Biochim Biophys Acta Date: 1951-03

17 in total

1. Metabolic basis for asthma and rhinitis: an integrated approach.

Authors: K P Agrawal; D Mehta; S Gupta; S K Chhabra
Journal: Lung Date: 1986 Impact factor: 2.584

2. Purification of normal human eosinophils using the different binding capacities of blood leucocytes for complexed rabbit IgG.

Authors: P C Tai; C J Spry
Journal: Clin Exp Immunol Date: 1977-05 Impact factor: 4.330

FORMATION OF CHARCOT-LEYDEN CRYSTALS IN HUMAN EOSINOPHILS AND BASOPHILS AND STUDY OF THE COMPOSITION OF ISOLATED CRYSTALS.

1. [Formation of crystals in basophelic leukocytes].

2. Multiple components in horse-radish peroxidase.

3. Histochemical studies of Charcot-Leyden crystals.

4. Protein measurement with the Folin phenol reagent.

5. [Demonstration by electrophoresis of the relation between ribonucleic acid, non-centrifugable at high speed, and cytoplasmic proteins].

6. Studies on Charcot-Leyden crystals.

7. ISOLATION OF GRANULES FROM EOSINOPHIL LEUCOCYTES AND STUDY OF THEIR ENZYME CONTENT.

8. The isolation and properties of the specific cytoplasmic granules of rabbit polymorphonuclear leucocytes.

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Review 3. Eosinophils. Assays and interpretation.

4. Comparative properties of the Charcot-Leyden crystal protein and the major basic protein from human eosinophils.

5. The antigen-induced degranulation of basophil leucocytes from atopic subjects, studied by phase-contrast microscopy.

6. Eosinophil cell separation from human peripheral blood.

7. Charcot-Leyden crystals and Curschmann spirals in asthmatic sputum.

8. Origin of crystalloid inclusions in macrophages II: evidence for derivation from eosinophil granulocyte breakdown.

9. Charcot-Leyden crystals. Formation from primate and lack of formation from nonprimate eosinophils.

10. Significance of eosinophilic granulocytes in chronic subdural hematomas.