Literature DB >> 14325287

COMPARISON OF THE ELUTION AND ADSORPTION OF PICORNAVIRUSES BY COTTON AND CALCIUM ALGINATE WOOL SWABS.

C A PHILLIPS, C A GRIM.   

Abstract

The recovery of picornaviruses by cotton and calcium alginate wool swabs was studied by use of prototype strains of poliovirus 1, echovirus 1, coxsackievirus A9, and rhinovirus 16/60. No significant differences in recovery of viruses by the two types of swabs could be demonstrated. It is suggested that use of cotton swabs in the virus laboratory be continued, since wool swabs may favor the recovery of undesirable bacterial contaminants. Adsorption of viruses by cotton and wool swabs was similar from virus-buffer mixtures at pH 4.0, 7.0, and 8.0 at 24 and 37 C. Elution of virus from cotton and wool swabs was also studied. There was no significant difference in the amount of virus eluted at pH 5.5, 7.1, or 8.4.

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Keywords:  ADSORPTION; ALGINATES; COTTON; COXSACKIE VIRUSES; ECHO VIRUSES; EXPERIMENTAL LAB STUDY; PICORNAVIRUS; POLIOVIRUS; RHINOVIRUS; TISSUE CULTURE; VIRUS CULTIVATION

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Year:  1965        PMID: 14325287      PMCID: PMC1058271          DOI: 10.1128/am.13.3.457-459.1965

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN: 0003-6919


  3 in total

1.  Factors influencing enterovirus and reovirus growth and plaque formation.

Authors:  C WALLIS; J L MELNICK; M BIANCHI
Journal:  Tex Rep Biol Med       Date:  1962

2.  The serial cultivation of human diploid cell strains.

Authors:  L HAYFLICK; P S MOORHEAD
Journal:  Exp Cell Res       Date:  1961-12       Impact factor: 3.905

3.  The use of calcium alginate soluble wool for the examination of cleansed eating utensils.

Authors:  R M CAIN; H STEELE
Journal:  Can J Public Health       Date:  1953-12
  3 in total
  1 in total

1.  Survival and detection of rotaviruses on environmental surfaces in day care centers.

Authors:  B H Keswick; L K Pickering; H L DuPont; W E Woodward
Journal:  Appl Environ Microbiol       Date:  1983-10       Impact factor: 4.792

  1 in total

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