AGAR-GEL PRECIPITIN-INHIBITION TECHNIQUE FOR C-REACTIVE PROTEIN DETERMINATIONS. 3. QUANTITATION OF C-REACTIVE PROTEIN IN SERUM SPECIMENS.

A quantitative C-reactive protein serological procedure has been developed. By use of this method, which is performed in agar-gel plates, from 2 to 654 mug of C-reactive protein per ml of titrated human serum can be detected. The method is based on the inhibition of a specific C-reactive protein antigen-antibody precipitate formed in agar-gel by the minimal reactive dilutions of each reagent in 48 hr. It is simple, sensitive, and readily reproducible.