THE CONTROL OF THE MEMBRANE POTENTIAL OF MUSCLE FIBERS BY THE SODIUM PUMP.

Frog sartorius muscles were made Na-rich by immersion in K-free sulfate Ringer's solution in the cold. The muscles were then loaded with Na(24) and the extracellular space cleared of radioactivity. When such Na-rich muscles were transferred to lithium sulfate Ringer's solution at 20 degrees C, Na efflux was observed to increase with time, to reach a maximum about 15 minutes after the transfer of the muscles to Li(2)SO(4), and then to decline. The decline in efflux from these muscles was proportional to ([Na](i))(8) over a considerable range of [Na](i). The membrane potential of Na-rich muscles was about -48 mv in K-free sulfate Ringer's at 4 degrees C but changed to -76 mv in the same solution at 20 degrees C and to -98 mv in Li(2)SO(4) Ringer's at 20 degrees C. By contrast, muscles with a normal [Na](i) showed a fall in membrane potential when transferred from K-free sulfate Ringer's to Li(2)SO(4) Ringer's solution. The general conclusions from this study are (a) that Na extrusion is capable of generating an electrical potential, and (b) that increases in [Na](i) lead to reversible increases in P(Na) of muscle fibers.