| Literature DB >> 1431600 |
S Sonnino1, D Acquotti, G Kirschner, A Uguaglianza, L Zecca, F Rubino, G Tettamanti.
Abstract
A simple procedure is described for preparing lyso-GM1, a GM1 derivative that lacks the fatty acid moiety, starting from GM1 ganglioside using a one-pot reaction. Ganglioside deacylation was carried out in KOH/propan-1-ol in the absence of oxygen. The yield of lyso-GM1 under optimal conditions (6 h, 90 degrees C, 0.2 N KOH, 1 mM GM1) was 54%. The chemical structure of lyso-GM1 was determined by 1H-NMR and FAB-MS analyses, thus proving that the acetamide groups of galactosamine and sialic acid units were not affected during the deacylation reaction.Entities:
Mesh：
Substances：
Year: 1992 PMID： 1431600
Source DB: PubMed Journal: J Lipid Res ISSN： 0022-2275 Impact factor: 5.922