Literature DB >> 1431162

Direct visualisation and quantification of cellular cytotoxicity using two colour flourescence.

B J Kroesen1, G Mesander, J G ter Haar, T H The, L de Leij.   

Abstract

A fluorescence method is described for the evaluation of cell death induced by cellular cytolytic activity. A green fluorescent membrane dye, D275, was used to label various target cell lines and propidium iodide (PI) uptake was used to assay cell death. Natural killer (NK), lymphokine activated killer (LAK) as well as targeted T cell mediated cellular cytotoxicity were quantified using the fluorescence method and compared to results obtained with the 51chromium (51Cr) release assay. A good correlation was found after an assay period of 4-8 h indicating that the fluorescence method is a reliable alternative to the 51Cr release assay.

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Year:  1992        PMID： 1431162     DOI： 10.1016/0022-1759(92)90009-i

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  11 in total

1.  Calcein-acetyoxymethyl cytotoxicity assay: standardization of a method allowing additional analyses on recovered effector cells and supernatants.

Authors:  S Neri; E Mariani; A Meneghetti; L Cattini; A Facchini
Journal:  Clin Diagn Lab Immunol       Date:  2001-11

2.  Effects of permeation enhancers on the transport of a peptidomimetic thrombin inhibitor (CRC 220) in a human intestinal cell line (Caco-2).

Authors:  U Werner; T Kissel; M Reers
Journal:  Pharm Res       Date:  1996-08       Impact factor: 4.200

3.  Rickettsiae stimulate dendritic cells through toll-like receptor 4, leading to enhanced NK cell activation in vivo.

Authors:  Jeffrey M Jordan; Michael E Woods; Lynn Soong; David H Walker
Journal:  J Infect Dis       Date:  2009-01-15       Impact factor: 5.226

4.  Identification of cells initiating human melanomas.

Authors:  Tobias Schatton; George F Murphy; Natasha Y Frank; Kazuhiro Yamaura; Ana Maria Waaga-Gasser; Martin Gasser; Qian Zhan; Stefan Jordan; Lyn M Duncan; Carsten Weishaupt; Robert C Fuhlbrigge; Thomas S Kupper; Mohamed H Sayegh; Markus H Frank
Journal:  Nature       Date:  2008-01-17       Impact factor: 49.962

5.  Antiviral drugs from the nucleoside analog family block volume-activated chloride channels.

Authors:  M Gschwentner; A Susanna; E Wöll; M Ritter; U O Nagl; A Schmarda; A Laich; G M Pinggera; H Ellemunter; H Huemer
Journal:  Mol Med       Date:  1995-05       Impact factor: 6.354

6.  A novel multiparametric flow cytometry-based cytotoxicity assay simultaneously immunophenotypes effector cells: comparisons to a 4 h 51Cr-release assay.

Authors:  G G Kim; V S Donnenberg; A D Donnenberg; W Gooding; T L Whiteside
Journal:  J Immunol Methods       Date:  2007-06-28       Impact factor: 2.303

7.  Synthesis and characterization of a photocleavable collagen-like peptide.

Authors:  Alfredo Ornelas; Kaitlyn N Williams; Kevin A Hatch; Aurelio Paez; Angela C Aguilar; Cameron C Ellis; Nishat Tasnim; Supriyo Ray; Carl W Dirk; Thomas Boland; Binata Joddar; Chunqiang Li; Katja Michael
Journal:  Org Biomol Chem       Date:  2018-02-07       Impact factor: 3.876

8.  Cytotoxic Efficiency of Human CD8+ T Cell Memory Subtypes.

Authors:  Arne Knörck; Gertrud Schäfer; Dalia Alansary; Josephine Richter; Lorenz Thurner; Markus Hoth; Eva C Schwarz
Journal:  Front Immunol       Date:  2022-04-13       Impact factor: 8.786

9.  Pharmacologic inhibition of protein phosphatase-2A achieves durable immune-mediated antitumor activity when combined with PD-1 blockade.

Authors:  Winson S Ho; Herui Wang; Dominic Maggio; John S Kovach; Qi Zhang; Qi Song; Francesco M Marincola; John D Heiss; Mark R Gilbert; Rongze Lu; Zhengping Zhuang
Journal:  Nat Commun       Date:  2018-05-29       Impact factor: 14.919

10.  Inhibitors Targeting CDK9 Show High Efficacy against Osimertinib and AMG510 Resistant Lung Adenocarcinoma Cells.

Authors:  Jaya Padmanabhan; Biswarup Saha; Chase Powell; Qianxing Mo; Bradford A Perez; Srikumar Chellappan
Journal:  Cancers (Basel)       Date:  2021-08-03       Impact factor: 6.575
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