Literature DB >> 14304254

HEAT STABILITY OF HUMAN PLACENTAL ALKALINE PHOSPHATASE.

F C NEALE, J S CLUBB, D HOTCHKIS, S POSEN.   

Abstract

Alkaline phosphatase prepared from human placentae shows greater resistance to heat inactivation than any other known alkaline phosphatase of human origin. In the presence of magnesium this enzyme may be heated at 70 degrees C. for 30 minutes without loss of activity whereas other human alkaline phosphatases lose most of their activity on being heated at 56 degrees C. for this period of time. This heat stability is seen in freshly prepared enzyme, in alcohol-fractionated and freeze-dried material, and in the sera of individuals into whom placental alkaline phosphatase has been infused. The clinical implications of our observations are briefly indicated.

Entities:  

Keywords:  ALKALINE PHOSPHATASE; BLOOD ALKALINE PHOSPHATASE; BLOOD CHEMICAL ANALYSIS; ELECTROPHORESIS; HEAT; HISTOCYTOCHEMISTRY; MAGNESIUM; PAGET'S DISEASE, EXTRA-MAMMARY; PLACENTA

Mesh：

Substances：

Year:  1965        PMID： 14304254      PMCID： PMC472943          DOI： 10.1136/jcp.18.3.359

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  16 in total

1.  THE MECHANISM OF THE ELEVATION OF SERUM ALKALINE PHOSPHATASE IN PREGNANCY.

Authors:  Y MCMASTER; R TENNANT; J S CLUBB; F C NEALE
Journal:  J Obstet Gynaecol Br Commonw       Date:  1964-10

2.  ETHANOL FRACTIONATION OF HUMAN SERUM ALKALINE PHOSPHATASE.

Authors:  A C PEACOCK; R A REED; E M HIGHSMITH
Journal:  Clin Chim Acta       Date:  1963-11       Impact factor: 3.786

3.  Serum alkaline phosphatase fractions in hepato-biliary and bone diseases.

Authors:  L CHIANDUSSI; S F GREENE; S SHERLOCK
Journal:  Clin Sci       Date:  1962-06       Impact factor: 6.124

4.  A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase.

Authors:  A GAREN; C LEVINTHAL
Journal:  Biochim Biophys Acta       Date:  1960-03-11

5.  Purification of placental alkaline phosphatase.

Authors:  Z AHMED; E J KING
Journal:  Biochim Biophys Acta       Date:  1960-05-20

6.  Comparison and combination of the starch-gel and filter-paper electrophoretic methods applied to human sera: two-dimensional electrophoresis.

Authors:  M D POULIK; O SMITHIES
Journal:  Biochem J       Date:  1958-04       Impact factor: 3.857

7.  MULTIPLE FORMS OF ENZYMES: TISSUE, ONTOGENETIC, AND SPECIES SPECIFIC PATTERNS.

Authors:  C L Markert; F Møller
Journal:  Proc Natl Acad Sci U S A       Date:  1959-05       Impact factor: 11.205

8.  Characterization of tissue alkaline phosphatases and their partial purification by starch-gel electrophoresis.

Authors:  D W MOSS; D M CAMPBELL; E ANAGNOSTOU-KAKARAS; E J KING
Journal:  Biochem J       Date:  1961-11       Impact factor: 3.857

9.  Microestimation of magnesium in body fluids.

Authors:  M ORANGE; H C RHEIN
Journal:  J Biol Chem       Date:  1951-03       Impact factor: 5.157

10.  A CONVENIENT METHOD FOR DETERMINING SERUM AND BILE PHOSPHATASE ACTIVITY.

Authors:  E J King; A R Armstrong
Journal:  Can Med Assoc J       Date:  1934-10       Impact factor: 8.262
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  30 in total

1.  A recombinant retrovirus encoding alkaline phosphatase confirms clonal boundary assignment in lineage analysis of murine retina.

Authors:  S C Fields-Berry; A L Halliday; C L Cepko
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-15       Impact factor: 11.205

2.  Catalytic properties and stability of three common variants of placental alkaline phosphatase.

Authors:  P A Holmgren; T Stigbrand
Journal:  Biochem Genet       Date:  1978-06       Impact factor: 1.890

3.  Characterization of pluripotent stem cells.

Authors:  Mercè Martí; Lola Mulero; Cristina Pardo; Cristina Morera; Meritxell Carrió; Leopoldo Laricchia-Robbio; Concepcion Rodriguez Esteban; Juan Carlos Izpisua Belmonte
Journal:  Nat Protoc       Date:  2013-01-10       Impact factor: 13.491

4.  Quantitative method for determining serum alkaline phosphatase isoenzyme activity II. Development and clinical application of method for measuring four serum alkaline phosphatase isoenzymes.

Authors:  M D Shephard; M J Peake; R N Walmsley
Journal:  J Clin Pathol       Date:  1986-09       Impact factor: 3.411

Review 5.  Alkaline phosphatase.

Authors:  T W Warnes
Journal:  Gut       Date:  1972-11       Impact factor: 23.059

6.  Maternal factors in neonatal hypocalcaemia: a study in three ethnic groups.

Authors:  P J Watney; G W Chance; P Scott; J M Thompson
Journal:  Br Med J       Date:  1971-05-22

7.  Evolution of alkaline phosphatases in primates.

Authors:  D J Goldstein; C Rogers; H Harris
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205

8.  Heat-stable alkaline phosphatase as a marker for human and monkey type-I pneumocytes.

Authors:  E J Nouwen; N Buyssens; M E De Broe
Journal:  Cell Tissue Res       Date:  1990-05       Impact factor: 5.249

9.  L-tryptophan. A non-allosteric organ-specific uncompetitive inhibitor of human placental alkaline phosphatase.

Authors:  C W Lin; H G Sie; W H Fishman
Journal:  Biochem J       Date:  1971-09       Impact factor: 3.857

10.  Human cell lines expressing intestinal alkaline phosphatase.

Authors:  F J Benham; H Harris
Journal:  Proc Natl Acad Sci U S A       Date:  1979-08       Impact factor: 11.205
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