Determination of ascorbic acid in elemental diet by high-performance liquid chromatography with electrochemical detection.

Determination of ascorbic acid in a multi-component elemental diet was performed by high-performance liquid chromatography with electrochemical detection. This method is suitable for the routine determination of ascorbic acid in elemental diet because it is simple, rapid, sensitive, highly selective and reproducible. The calibration graph of ascorbic acid was linear in the range 0-1.0 micrograms. The recovery of ascorbic acid was over 95% by the standard addition method. There was good agreement between the concentrations of ascorbic acid stated and found.