EFFECT OF VARIOUS CONDITIONS ON ACCUMULATION OF OXYTETRACYCLINE IN ESCHERICHIA COLI.

Izaki, Kazuo (University of Tokyo, Tokyo, Japan), and Kei Arima. Effect of various conditions on accumulation of oxytetracycline in Escherichia coli. J. Bacteriol. 89:1335-1339. 1965.-Accumulation of large amounts of oxytetracycline occurred in Escherichia coli when the cells were incubated with high concentrations of oxytetracycline (100 to 400 mug/ml) in nutrient broth or in a medium containing glucose, K(2)HPO(4), and MgSO(4). In the absence of glucose or MgSO(4), the accumulation was very small. The optimal pH for accumulation was about 6.5. Manganous ion could replace Mg(++) in promoting the accumulation, though with decreased effectiveness. Malate and succinate were effective promoters of accumulation as well as glucose. Accumulation was inhibited at low temperatures or in the presence of metabolic inhibitors such as 2,4-dinitrophenol or sodium azide.