Literature DB >> 14290949

HOG CHOLERA. II. RELIABILITY OF THE AGAR DOUBLE DIFFUSION PRECIPITATION TEST FOR THE DIFFERENTIATION OF H.C. VIRUS FROM OTHER INFECTIOUS AGENTS IN SWINE TISSUE.

G M RUCKERBAUER, M APPEL, D P GRAY, G L BANNISTER, P BOULANGER.   

Abstract

The specificity in the agar diffusion precipitation test of the reaction between the antigen of hog cholera virus diffusing from infected tissues and its homologous antibody was verified. Alternate freezing and thawing of infected tissues was found to give optimum release of the antigen from fresh tissue frozen for 18 hours. A study of the effect of the size and age of pigs upon the diffusion of the antigen from tissues showed that tissues from pigs of less than 250 lbs. gave good results provided the tissues were from animals showing gross clinical manifestations. Specimens from infected breeding sows and dead animals usually did not give a reaction.

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Keywords:  DIAGNOSIS, DIFFERENTIAL; DIAGNOSIS, LABORATORY; ERYSIPELOTHRIX; FEVER; GEL DIFFUSION TESTS; HOG CHOLERA; SERUM GLOBULINS

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Year:  1965        PMID： 14290949      PMCID： PMC1494412     

Source DB:  PubMed          Journal:  Can J Comp Med Vet Sci        ISSN： 0316-5957


  1 in total

1.  Hog Cholera: I. Investigation of the Agar Double-Diffusion Precipitation Test for The Detection of the Virus in Swine Tissue.

Authors:  G M Ruckerbauer; M Appel; G L Bannister; K Mori; D Cochrane; P Boulanger
Journal:  Can J Comp Med Vet Sci       Date:  1964-12
  1 in total
  1 in total

1.  African swine fever. 3. The use of the agar double-diffusion precipitation test for the detection of the virus in swine tissue.

Authors:  P Boulanger; G L Bannister; D P Gray; G M Ruckerbauer; N G Willis
Journal:  Can J Comp Med Vet Sci       Date:  1967-01
  1 in total

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