| Literature DB >> 14280441 |
Abstract
A hemagglutination method employing tanned human O erythrocytes sensitized with purified Group A streptococcal polysaccharide has been developed to measure A polysaccharide antibody in antistreptococcal rabbit and human sera. The reaction of sensitized RBC with known Group A streptococcal rabbit antisera, inhibition of hemagglutination with A polysaccharide, and abolition of hemagglutination and hemagglutination-inhibition by exposure of polysaccharide antigen to A enzyme, which destroys the specific immunologic determinant of A carbohydrate, were utilized to demonstrate the specificity of this hemagglutinating system. The results of A polysaccharide antibody determinations on sera from normal persons, patients convalescent from streptococcal infection, and patients with non-suppurative streptococcal sequelae are presented.Entities:
Keywords: ANTIBODIES; CARRIER STATE; CHILD; CHROMATOGRAPHY; EXPERIMENTAL LAB STUDY; HEMAGGLUTINATION; HEMAGGLUTINATION INHIBITION TESTS; NEPHRITIS; POLYSACCHARIDES, BACTERIAL; RABBITS; RHEUMATIC FEVER; SEPTICEMIA; STREPTOCOCCAL INFECTIONS; STREPTOCOCCUS
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Year: 1965 PMID: 14280441 PMCID: PMC2138003 DOI: 10.1084/jem.121.5.793
Source DB: PubMed Journal: J Exp Med ISSN: 0022-1007 Impact factor: 14.307