Literature DB >> 1426252

Biochemical properties of rat protein kinase C-eta expressed in COS cells.

L V Dekker1, P J Parker, P McIntyre.   

Abstract

Using a PKC-epsilon cDNA probe a cDNA for PKC-eta has been cloned from a rat lung cDNA library. When expressed in COS cells, rat PKC-eta appeared as an 84 kDa protein. PKC-eta expressed in COS cells, was solubilized by 1% Triton X-100 and purified away from the endogenous PKC-alpha by ammonium sulphate fractionation. The activity of this PKC-eta preparation was characterized with respect to cofactor dependence and substrate specificity. Various PKC pseudosubstrate peptides are phosphorylated by PKC-eta in a phospholipid and TPA-dependent but calcium-independent manner. The polypeptide histone IIIS is a poor substrate.

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Year:  1992        PMID： 1426252     DOI： 10.1016/0014-5793(92)80934-9

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  10 in total

1.  Role of protein kinase C in the inhibitory action of trophoblast interferons on expression of the oxytocin receptor in sheep endometrium.

Authors:  D R Abayasekara; E L Sheldrick; H C Flick-Smith; A P Flint
Journal:  Endocrine       Date:  1995-02       Impact factor: 3.633

2.  The eta isoform of protein kinase C is localized on rough endoplasmic reticulum.

Authors:  K Chida; H Sagara; Y Suzuki; A Murakami; S Osada; S Ohno; K Hirosawa; T Kuroki
Journal:  Mol Cell Biol       Date:  1994-06       Impact factor: 4.272

Review 3.  Protein kinase C isoenzymes: divergence in signal transduction?

Authors:  H Hug; T F Sarre
Journal:  Biochem J       Date:  1993-04-15       Impact factor: 3.857

4.  Localization of non-conventional protein kinase C isoforms in bovine brain cell nuclei.

Authors:  U Rosenberger; M Shakibaei; K Buchner
Journal:  Biochem J       Date:  1995-01-01       Impact factor: 3.857

5.  Expression of mammalian protein kinase C in Schizosaccharomyces pombe: isotype-specific induction of growth arrest, vesicle formation, and endocytosis.

Authors:  N T Goode; M A Hajibagheri; G Warren; P J Parker
Journal:  Mol Biol Cell       Date:  1994-08       Impact factor: 4.138

6.  Partial purification of a type eta protein kinase C from murine brain: separation from other protein kinase C isoenzymes and characterization.

Authors:  R Zang; H J Müller; K Kielbassa; F Marks; M Gschwendt
Journal:  Biochem J       Date:  1994-12-01       Impact factor: 3.857

7.  Threonine-497 is a critical site for permissive activation of protein kinase C alpha.

Authors:  S Cazaubon; F Bornancin; P J Parker
Journal:  Biochem J       Date:  1994-07-15       Impact factor: 3.857

8.  Interferon alpha induces protein kinase C-epsilon (PKC-epsilon) gene expression and a 4.7-kb PKC-epsilon-related transcript.

Authors:  C Wang; S N Constantinescu; D J MacEwan; B Strulovici; L V Dekker; P J Parker; L M Pfeffer
Journal:  Proc Natl Acad Sci U S A       Date:  1993-08-01       Impact factor: 11.205

9.  Protein kinase C isoforms in bovine aortic endothelial cells: role in regulation of P2Y- and P2U-purinoceptor-stimulated prostacyclin release.

Authors:  V Patel; C Brown; M R Boarder
Journal:  Br J Pharmacol       Date:  1996-05       Impact factor: 8.739

10.  Localization of mRNAs for novel, atypical as well as conventional protein kinase C (PKC) isoforms in the brain of developing and mature rats.

Authors:  H Minami; Y Owada; R Suzuki; Y Handa; H Kondo
Journal:  J Mol Neurosci       Date:  2000-10       Impact factor: 2.866
  10 in total

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