Literature DB >> 1426246

Expression of functional Thermoplasma acidophilum proteasomes in Escherichia coli.

P Zwickl1, F Lottspeich, W Baumeister.   

Abstract

The two genes encoding the constituent subunits of the Thermoplasma acidophilum proteasome were expressed in Escherichia coli yielding fully assembled molecules as shown by electron microscopy. The recombinant proteasomes were purified to homogeneity and were shown to have proteolytic activity indistinguishable from proteasomes isolated from T. acidophilum.

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Year:  1992        PMID: 1426246     DOI: 10.1016/0014-5793(92)80925-7

Source DB:  PubMed          Journal:  FEBS Lett        ISSN: 0014-5793            Impact factor:   4.124


  18 in total

Review 1.  The proteasome: a macromolecular assembly designed for controlled proteolysis.

Authors:  P Zwickl; D Voges; W Baumeister
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  1999-09-29       Impact factor: 6.237

2.  A voyage to the inner space of cells.

Authors:  Wolfgang Baumeister
Journal:  Protein Sci       Date:  2005-01       Impact factor: 6.725

3.  Role of the beta1 subunit in the function and stability of the 20S proteasome in the hyperthermophilic archaeon Pyrococcus furiosus.

Authors:  Lara S Madding; Joshua K Michel; Keith R Shockley; Shannon B Conners; Kevin L Epting; Matthew R Johnson; Robert M Kelly
Journal:  J Bacteriol       Date:  2006-11-17       Impact factor: 3.490

4.  Purification and Characterization of a Proteasome from the Hyperthermophilic Archaeon Pyrococcus furiosus.

Authors:  M W Bauer; S H Bauer; R M Kelly
Journal:  Appl Environ Microbiol       Date:  1997-03       Impact factor: 4.792

Review 5.  Structure and structure formation of the 20S proteasome.

Authors:  M Schmidt; G Schmidtke; P M Kloetzel
Journal:  Mol Biol Rep       Date:  1997-03       Impact factor: 2.316

6.  Molecular biology of extremophiles.

Authors:  M Ciaramella; R Cannio; M Moracci; F M Pisani; M Rossi
Journal:  World J Microbiol Biotechnol       Date:  1995-01       Impact factor: 3.312

7.  Sequence, expression in Escherichia coli, and analysis of the gene encoding a novel intracellular protease (PfpI) from the hyperthermophilic archaeon Pyrococcus furiosus.

Authors:  S B Halio; I I Blumentals; S A Short; B M Merrill; R M Kelly
Journal:  J Bacteriol       Date:  1996-05       Impact factor: 3.490

Review 8.  Structural features of archaebacterial and eukaryotic proteasomes.

Authors:  A J Koster; J Walz; A Lupas; W Baumeister
Journal:  Mol Biol Rep       Date:  1995       Impact factor: 2.316

9.  Proteolytic systems of archaea: slicing, dicing, and mincing in the extreme.

Authors:  Julie A Maupin-Furlow
Journal:  Emerg Top Life Sci       Date:  2018-11-14

Review 10.  Archaeal proteasomes and sampylation.

Authors:  Julie A Maupin-Furlow
Journal:  Subcell Biochem       Date:  2013
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