Literature DB >> 14250329

SYNTHESIS OF BLOCK OLIGONUCLEOTIDES.

R E THACH, P DOTY.   

Abstract

The concentration of sodium chloride strongly influences primed polymerization of nucleoside diphosphates by polynucleotide phosphorylase. High concentrations of NaCl allow the addition of only a few nucleotide residues onto the 3' end of the primer. When pure oligonucleotides are used as primers and when the product is fractionated, pure block oligonucleotides can be obtained.

Entities:  

Keywords:  CARBON ISOTOPES; CHEMISTRY; CHROMATOGRAPHY; EXPERIMENTAL LAB STUDY; MAGNESIUM; NUCLEOTIDES; NUCLEOTIDYLTRANSFERASES; SODIUM CHLORIDE; SPECTROPHOTOMETRY

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Substances：

Year:  1965        PMID： 14250329     DOI： 10.1126/science.147.3663.1310

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  7 in total

1.  Translation of the genetic message: factors involved in the initiation of protein synthesis.

Authors:  W M Stanley; M Salas; A J Wahba; S Ochoa
Journal:  Proc Natl Acad Sci U S A       Date:  1966-07       Impact factor: 11.205

2.  The binding of aminoacyl-sRNA's to ribosomes stimulated by block oligonucleotides.

Authors:  R E Thach; T A Sundararajan
Journal:  Proc Natl Acad Sci U S A       Date:  1965-05       Impact factor: 11.205

3.  Direction of reading of the genetic message. II.

Authors:  M A Smith; M Salas; W M Stanley; A J Wahba; S Ochoa
Journal:  Proc Natl Acad Sci U S A       Date:  1966-01       Impact factor: 11.205

4.  The polarity of messenger translation in protein synthesis.

Authors:  R E Thach; M A Cecere; T A Sundararajan; P Doty
Journal:  Proc Natl Acad Sci U S A       Date:  1965-10       Impact factor: 11.205

5.  Polynucleotides. XLII1. Limited addition of 2'O-onitrobenzyl nucleotides to the 3'-end of ribooligonucleotide with polynucleotide phosphorylase.

Authors:  M Ikehara; S Tanaka; T Fukui; E Ohtsuka
Journal:  Nucleic Acids Res       Date:  1976-11       Impact factor: 16.971

6.  Base substitutions in the wobble position of the anticodon inhibit aminoacylation of E. coli tRNAfMet by E. coli Met-tRNA synthetase.

Authors:  L H Schulman; H Pelka; M Susani
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

7.  Anticodon loop size and sequence requirements for recognition of formylmethionine tRNA by methionyl-tRNA synthetase.

Authors:  L H Schulman; H Pelka
Journal:  Proc Natl Acad Sci U S A       Date:  1983-11       Impact factor: 11.205
  7 in total

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