Literature DB >> 14247690

QUANTITATIVE SEPARATION OF RADIOACTIVE STEROLS AND BILE ACIDS IN HUMAN FAECES.

B LEWIS, N B MYANT.   

Abstract

A method is described for the quantitative separation of radioactive bile acids and neutral sterols in human faeces on a potassium hydroxide-treated silicic acid column. Results obtained with faecal extracts containing metabolites derived from radioactive cholesterol, cholic acid, and chenodeoxycholic acid show that the efficiency of extraction is at least 96% and that mutual contamination between the bile-acid and sterol fractions is between 0.7 and 3.8%. The method gives better results than one based on separation of the faecal steroids into saponifiable and unsaponifiable material.

Entities:  

Keywords:  BILE ACIDS AND SALTS; CARBON ISOTOPES; CHOLESTEROL; CHROMATOGRAPHY; FECES; TRITIUM

Mesh:

Substances:

Year:  1965        PMID: 14247690      PMCID: PMC472841          DOI: 10.1136/jcp.18.1.105

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN: 0021-9746            Impact factor:   3.411


  10 in total

1.  THIN-LAYER CHROMATOGRAPHY OF BILE ACIDS.

Authors:  P ENEROTH
Journal:  J Lipid Res       Date:  1963-01       Impact factor: 5.922

2.  On the turnover and excretory products of cholic and chenodeoxycholic acid in man.

Authors:  H DANIELSSON; P ENEROTH; K HELLSTROM; S LINDSTEDT; J SJOVALL
Journal:  J Biol Chem       Date:  1963-07       Impact factor: 5.157

3.  Formation of trihydroxycoprostanic acid from cholesterol in man.

Authors:  E STAPLE; J L RABINOWITZ
Journal:  Biochim Biophys Acta       Date:  1962-06-04

4.  The influence of diet on fecal lipids in South African white and Bantu prisoners.

Authors:  A Antonis; I Bersohn
Journal:  Am J Clin Nutr       Date:  1962-08       Impact factor: 7.045

5.  The preparation of serum lipoproteins labeled with radioactive cholesterol.

Authors:  A F WHEREAT; E STAPLE
Journal:  Arch Biochem Biophys       Date:  1960-10       Impact factor: 4.013

6.  Dietary fat and cholesterol metabolism; faecal elimination of bile acids and other lipids.

Authors:  H GORDON; B LEWIS; L EALES; J F BROCK
Journal:  Lancet       Date:  1957-12-28       Impact factor: 79.321

7.  On the separation of bile acids by partition chromatography.

Authors:  J SJOVALL
Journal:  Acta Physiol Scand       Date:  1953-10-06

8.  Excretion of steroid acids in man.

Authors:  R S ROSENFELD; L HELLMAN
Journal:  Arch Biochem Biophys       Date:  1962-05       Impact factor: 4.013

9.  In vitro formation of deoxycholic and lithocholic acid by human intestinal microorganisms.

Authors:  A NORMAN; M S SHORB
Journal:  Proc Soc Exp Biol Med       Date:  1962-07

Review 10.  Factors influencing formation and excretion of bile acids.

Authors:  S Bergström; H Danielsson
Journal:  Biochem Soc Symp       Date:  1963
  10 in total
  1 in total

1.  Absorption of vitamin D3-3H in control subjects and patients with intestinal malabsorption.

Authors:  G R Thompson; B Lewis; C C Booth
Journal:  J Clin Invest       Date:  1966-01       Impact factor: 14.808

  1 in total

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