| Literature DB >> 14247690 |
Abstract
A method is described for the quantitative separation of radioactive bile acids and neutral sterols in human faeces on a potassium hydroxide-treated silicic acid column. Results obtained with faecal extracts containing metabolites derived from radioactive cholesterol, cholic acid, and chenodeoxycholic acid show that the efficiency of extraction is at least 96% and that mutual contamination between the bile-acid and sterol fractions is between 0.7 and 3.8%. The method gives better results than one based on separation of the faecal steroids into saponifiable and unsaponifiable material.Entities:
Keywords: BILE ACIDS AND SALTS; CARBON ISOTOPES; CHOLESTEROL; CHROMATOGRAPHY; FECES; TRITIUM
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Year: 1965 PMID: 14247690 PMCID: PMC472841 DOI: 10.1136/jcp.18.1.105
Source DB: PubMed Journal: J Clin Pathol ISSN: 0021-9746 Impact factor: 3.411