| Literature DB >> 1423723 |
E A Hosford1, H Sone, J Tanaka.
Abstract
YEp plasmid stability in the presence of either Saccharomyces cerevisiae laboratory strain 2-microns DNA, or lager brewing yeast 2-microns DNA in the same genetic background, was compared under non-selective culture conditions. It was found that YEp plasmids were more stably maintained in the presence of lager 2-microns DNA under these conditions. By construction of laboratory-lager 2-microns DNA hybrid plasmids, an 867 bp StuI fragment of lager 2-microns DNA was shown to be responsible for the enhanced stability of the YEp plasmid. Nucleotide substitutions at two sites were found by sequencing this region. It was also confirmed that increasing cell ploidy enhanced YEp stability under non-selective conditions.Entities:
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Year: 1992 PMID： 1423723 DOI： 10.1007/bf00352436
Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886