| Literature DB >> 1421766 |
A M Ryan1, D S Gallagher, J E Womack.
Abstract
The previous assignment of bovine alpha-(IFNA) and beta-(IFNB) interferon gene families to syntenic group U18 was confirmed with additional cDNA probes and a bovine-rodent hybrid somatic cell panel representing all 29 bovine autosomal syntenic groups. Fluorescent in situ hybridization (FISH) localized these genes to bovine Chromosome (Chr) 8 band 15 and demonstrates that with biotinylated plasmids, as few as five tandemly arrayed sequences can be detected by conventional fluorescent microscopy. This technique can be applied to physical mapping of other multicopy genes in domestic animals.Entities:
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Year: 1992 PMID: 1421766 DOI: 10.1007/bf00350624
Source DB: PubMed Journal: Mamm Genome ISSN: 0938-8990 Impact factor: 2.957