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Literature DB >> 14191707

ANTIBODY FORMATION IN VITRO BY SEPARATED SPLEEN CELLS: INHIBITION BY ACTINOMYCIN OR CHLORAMPHENICOL.

Abstract

The formation of 19S antibody to poliovirus by rabbit spleen cells in vitro was interrupted by adding actinomycin D (1 to 10 micrograms per milliliter) for 30 minutes or longer. Shorter treatment (5 to 15 minutes) caused partial suppression. Antibody formation was slowly renewed upon removal of the drug. These results suggest that 19S antibody formation is contingent upon DNA-dependent RNA synthesis and that the genetic messenger has an average lifetime of one-half day or less. Treatment with chloramphenicol (20 to 50 micrograms per milliliter) for 3 days caused the cessation of 19S antibody formation.

Entities: Chemical Species

Keywords: ACTINOMYCIN; ANTIBODY FORMATION; ANTIGEN-ANTIBODY REACTIONS; CHLORAMPHENICOL; EXPERIMENTAL LAB STUDY; GAMMA GLOBULIN, 19S; PHARMACOLOGY; POLIOVIRUS; RABBITS; SPLEEN

Mesh：

Substances：

Year: 1964 PMID： 14191707 DOI： 10.1126/science.146.3644.659

Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728

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Cited

13 in total

1. IN VITRO SECONDARY 19S AND 7S ANTIBODY RESPONSES TO POLIOVIRUS IN MEMBRANE CULTURES OF SEPARATED SPLEEN CELLS.

Authors: S E SVEHAG
Journal: Arch Gesamte Virusforsch Date: 1965

2. Kinetics of antibody production by single cells. II. The action of transcription and translation inhibitors upon the metabolism of haemolysin-secreting cells.

Authors: J Weyer; A E Bussard
Journal: Immunology Date: 1972-06 Impact factor: 7.397

3. Different effects of chloramphenicol, dactinomycin, and streptovitacin A on synthesis of tumor and virion antigens in SV40 virus-infected cells.

Authors: A B Sabin
Journal: Proc Natl Acad Sci U S A Date: 1966-05 Impact factor: 11.205

Review 4. [The amyloidoses].

Authors: G Bruns
Journal: Klin Wochenschr Date: 1967-09-01

Review 5. Symposium on in vitro studies of the immune response. IV. Role of nucleic acids in the anamnestic antibody response.

Authors: A B Stavitsky; J P Gusdon
Journal: Bacteriol Rev Date: 1966-06

ANTIBODY FORMATION IN VITRO BY SEPARATED SPLEEN CELLS: INHIBITION BY ACTINOMYCIN OR CHLORAMPHENICOL.

1. IN VITRO SECONDARY 19S AND 7S ANTIBODY RESPONSES TO POLIOVIRUS IN MEMBRANE CULTURES OF SEPARATED SPLEEN CELLS.

2. Kinetics of antibody production by single cells. II. The action of transcription and translation inhibitors upon the metabolism of haemolysin-secreting cells.

3. Different effects of chloramphenicol, dactinomycin, and streptovitacin A on synthesis of tumor and virion antigens in SV40 virus-infected cells.

Review 4. [The amyloidoses].

Review 5. Symposium on in vitro studies of the immune response. IV. Role of nucleic acids in the anamnestic antibody response.

6. The effect of Streptomycin on antibody synthesis in vitro.

7. [Antibody synthesis in vitro. II. Stimulation of a cell-free system].

8. Two classes of spleen ribosomes with different sensitivities to chloramphenicol.

9. Some metabolic aspects of tolerance to bacterial endotoxin.

10. Primary antibody response studied by using inhibitors of nucleic acid synthesis.