Literature DB >> 1418976

Periodic acid/Schiff staining of glycoproteins immobilized on a blotting matrix.

M Strömqvist1, H Gruffman.   

Abstract

A simple and sensitive method for the detection of glycoproteins and glycopeptides in solution and in polyacrylamide gels is described. This method combines the well-known periodic acid/Schiff stain with protein blotting. Compared with direct staining of a polyacrylamide gel, the sensitivity is considerably increased. Using a set of glycoproteins, we have found the sensitivity to be about 4 ng carbohydrate.

Entities:  

Mesh：

Substances：

Year:  1992        PMID： 1418976

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  13 in total

1.  Molecular control of acid phosphatase secretion into the rhizosphere of proteoid roots from phosphorus-stressed white lupin.

Authors:  S S Miller; J Liu; D L Allan; C J Menzhuber; M Fedorova; C P Vance
Journal:  Plant Physiol       Date:  2001-10       Impact factor: 8.340

2.  Cloning and targeted disruption of MLG1, a gene encoding two of three extracellular mixed-linked glucanases of Cochliobolus carbonum.

Authors:  J M Görlach; E Van Der Knaap; J D Walton
Journal:  Appl Environ Microbiol       Date:  1998-02       Impact factor: 4.792

3.  A novel function for the cathepsin D inhibitor in tomato.

Authors:  Purificación Lisón; Ismael Rodrigo; Vicente Conejero
Journal:  Plant Physiol       Date:  2006-09-29       Impact factor: 8.340

4.  Cloning, mutagenesis, and structural analysis of human pancreatic alpha-amylase expressed in Pichia pastoris.

Authors:  E H Rydberg; G Sidhu; H C Vo; J Hewitt; H C Côte; Y Wang; S Numao; R T MacGillivray; C M Overall; G D Brayer; S G Withers
Journal:  Protein Sci       Date:  1999-03       Impact factor: 6.725

5.  The chemotactic response of Vibrio anguillarum to fish intestinal mucus is mediated by a combination of multiple mucus components.

Authors:  R O'Toole; S Lundberg; S A Fredriksson; A Jansson; B Nilsson; H Wolf-Watz
Journal:  J Bacteriol       Date:  1999-07       Impact factor: 3.490

6.  Targeted mutants of Cochliobolus carbonum lacking the two major extracellular polygalacturonases.

Authors:  J S Scott-Craig; Y Q Cheng; F Cervone; G De Lorenzo; J W Pitkin; J D Walton
Journal:  Appl Environ Microbiol       Date:  1998-04       Impact factor: 4.792

7.  Evaluation of receptor binding specificity of Escherichia coli K88 (F4) fimbrial adhesin variants using porcine serum transferrin and glycosphingolipids as model receptors.

Authors:  Philippe A Grange; Michèle A Mouricout; Steven B Levery; David H Francis; Alan K Erickson
Journal:  Infect Immun       Date:  2002-05       Impact factor: 3.441

8.  Characterization of porcine intestinal receptors for the K88ac fimbrial adhesin of Escherichia coli as mucin-type sialoglycoproteins.

Authors:  A K Erickson; D R Baker; B T Bosworth; T A Casey; D A Benfield; D H Francis
Journal:  Infect Immun       Date:  1994-12       Impact factor: 3.441

9.  Streptomyces lividans glycosylates the linker region of a beta-1,4-glycanase from Cellulomonas fimi.

Authors:  E Ong; D G Kilburn; R C Miller; R A Warren
Journal:  J Bacteriol       Date:  1994-02       Impact factor: 3.490

10.  A component of the medicinal herb ephedra blocks activation in the classical and alternative pathways of complement.

Authors:  M Ling; S J Piddlesden; B P Morgan
Journal:  Clin Exp Immunol       Date:  1995-12       Impact factor: 4.330
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.