Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 TYPING OF GROUP A STREPTOCOCCI BY IMMUNOFLUORESCENCE. I. PREPARATION AND PROPERTIES OF TYPE I FLUORESCEIN-LABELED ANTIBODY.

Literature DB >> 14188716

TYPING OF GROUP A STREPTOCOCCI BY IMMUNOFLUORESCENCE. I. PREPARATION AND PROPERTIES OF TYPE I FLUORESCEIN-LABELED ANTIBODY.

W W KARAKAWA, E K BORMAN, C R MCFARLAND.

Abstract

Karakawa, Walter W. (Connecticut State Department of Health, Hartford), Earle K. Borman, and Clarence R. McFarland. Typing of group A streptococci by immunofluorescence. I. Preparation and properties of type 1 fluorescein-labeled antibody. J. Bacteriol. 87:1377-1382. 1964.-Unadsorbed, fluorescein-labeled globulins derived from rabbits immunized with acid-extracted M-protein of type 1 streptococci (plus adjuvant) were found to have high fluorescent-antibody (FA) staining titers and to be considerably more type-specific than were similar preparations derived from whole-cell immunization. Appropriate adsorption rendered the anti-M reagent entirely type-specific without appreciable loss of titer; whole-cell reagent was appreciably weakened in FA titer by comparable adsorption. Type-specificity was confirmed by parallel bactericidal, long-chain, and precipitin studies. Removal of reactivity by adsorption with homologous M protein was complete, confirming that the FA reaction was truly a manifestation of an M anti-M protein system. The data indicate that the development of FA reagents specific for the streptococcal types is feasible.

Entities: Chemical Species

Keywords: BACTERIOLOGICAL TECHNICS; EXPERIMENTAL LAB STUDY; FLUORESCENT ANTIBODY TECHNIC; STREPTOCOCCUS

Mesh：

Substances：
Fluorescein

Year: 1964 PMID： 14188716 PMCID： PMC277213 DOI： 10.1128/jb.87.6.1377-1382.1964

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

10 in total

1. Studies of streptococcal cell walls. VI. Effects of adjuvants on the production of type-specific antibodies to cell walls and isolated M protein.

Authors: J A HAYASHI; G WALSH
Journal: J Bacteriol Date: 1961-11 Impact factor: 3.490

2. Superiority of fluorescein isothiocyanate (Riggs) for fluorescent-antibody technic with a modification of its application.

Authors: J D MARSHALL; W C EVELAND; C W SMITH
Journal: Proc Soc Exp Biol Med Date: 1958 Aug-Sep

8. Preparation and antigenicity of M protein released from group A, type 1 streptococcal cell walls by phage-associated lysin.

Authors: F S KANTOR; R M COLE
Journal: J Exp Med Date: 1960-07-01 Impact factor: 14.307

9. Localization of streptococcal antigens in tissues. I. Histologic distribution and persistence of M protein, types 1, 5, 12, and 19 in the tissues of the mouse.

Authors: M H KAPLAN
Journal: J Exp Med Date: 1958-03-01 Impact factor: 14.307

10. Long chain formation by strains of group A streptococci in the presence of homologous antiserum: a type-specific reaction.

Authors: G H STOLLERMAN; R EKSTEDT
Journal: J Exp Med Date: 1957-09-01 Impact factor: 14.307

10 in total

11 in total

Review 4. M proteins of group A streptococci.

Authors: E N Fox
Journal: Bacteriol Rev Date: 1974-03

5. A direct fluorescent antibody test for identification of group E streptococci.

Authors: R L Schueler; L G Morehouse; L D Olson
Journal: Can J Comp Med Date: 1973-10

6. [Clinical use of immunofluorescence. II. Preparation of the reagents needed for the immunofluorescence method and localization of bacteria in the tissues].

Authors: T B Smith; B Heymer; O Haferkamp
Journal: Z Med Mikrobiol Immunol Date: 1966

TYPING OF GROUP A STREPTOCOCCI BY IMMUNOFLUORESCENCE. I. PREPARATION AND PROPERTIES OF TYPE I FLUORESCEIN-LABELED ANTIBODY.

1. Studies of streptococcal cell walls. VI. Effects of adjuvants on the production of type-specific antibodies to cell walls and isolated M protein.

2. Superiority of fluorescein isothiocyanate (Riggs) for fluorescent-antibody technic with a modification of its application.

3. Studies of streptococcal cell walls. I. Isolation, chemical composition, and preparation of M protein.

4. Staining bacterial smears with fluorescent antibody. IV. Grouping streptococci with fluorescent antibody.

5. Determination of cerebrospinal fluid protein with the Folin phenol reagent.

6. Studies of phagocytosis of group A streptococci by polymorphonuclear leucocytes in vitro.

7. Preparation and properties of type-specific M antigen isolated from a group A, type 1 hemolytic streptococcus.

8. Preparation and antigenicity of M protein released from group A, type 1 streptococcal cell walls by phage-associated lysin.

9. Localization of streptococcal antigens in tissues. I. Histologic distribution and persistence of M protein, types 1, 5, 12, and 19 in the tissues of the mouse.

10. Long chain formation by strains of group A streptococci in the presence of homologous antiserum: a type-specific reaction.

1. CLINICAL APPLICATION OF IMMUNOFLUORESCENCE. I. GROUPING BETA-HEMOLYTIC STREPTOCOCCI.

Review 2. FLUORESCENT-ANTIBODY TECHNIQUES IN DIAGNOSTIC BACTERIOLOGY.

3. Diversity dynamics of marine bacteria studied by immunofluorescent staining on membrane filters.

Review 4. M proteins of group A streptococci.

5. A direct fluorescent antibody test for identification of group E streptococci.

6. [Clinical use of immunofluorescence. II. Preparation of the reagents needed for the immunofluorescence method and localization of bacteria in the tissues].

7. Immunofluorescent identification of Type 12 group A streptococci.

8. Heterogeneity of group A type-specific antibodies.

9. Assay of type-specific M antigens on whole group A streptococci.

10. Clinical application of immunofluorescence. 3. Identification of Lancefield group B streptococci.