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Literature DB >> 14185317

PHYTOHEMAGGLUTININ ELICITATION OF SPECIFIC ANAMNESTIC IMMUNE RESPONSE IN VITRO.

Abstract

Fragments of lymph nodes from rabbits hyperimmunized with human chorionic gonadotropin or bovine plasma albumin were cultivated in organ culture for 1 month. An anamnestic response was elicited in vitro by the sensitizing antigen. Phytohemagglutinin also produced such a response. Throughout cultivation the cultures showed excellent preservation of morphological integrity and of capacity to produce antibody.

Entities: Species

Keywords: ANTIGEN-ANTIBODY REACTIONS; EXPERIMENTAL LAB STUDY; GONADOTROPINS, CHORIONIC; LYMPH NODES; PHARMACOLOGY; PLANT AGGLUTININS; RABBITS; TISSUE CULTURE

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Year: 1964 PMID： 14185317 DOI： 10.1126/science.146.3641.247

Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728

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Cited

11 in total

Review 1. [Lymphocyte culture and its relation to clinical problems].

Authors: E Ambs
Journal: Klin Wochenschr Date: 1969-08-01

2. Immunoglobulin synthesis by human peripheral lymphocytes and thymocytes in vitro. Specificity of immunochemical methods and stimulation by phytohaemagglutinin.

Authors: I J Forbes; J L Smith
Journal: Clin Exp Immunol Date: 1969-12 Impact factor: 4.330

3. Affinity of antigen for white cells and its relation to the induction of antibody formation.

Authors: D Sulitzeanu
Journal: Bacteriol Rev Date: 1968-12

Review 4. Symposium on in vitro studies of the immune response. II. Significance of the reaction of lymphoid cells to homologous tissue.

Authors: R W Dutton
Journal: Bacteriol Rev Date: 1966-06

10. Studies on rabbit lymphocytes in vitro. 3. Proteins, RNA, and DNA synthesis by lymphocyte cultures after stimulation with phytohaemagglutinin, with staphylococcal filtrate, with antiallotype serum, and with heterologous antiserum to rabbit whole serum.

Authors: S Sell; D S Rowe; P G Gell
Journal: J Exp Med Date: 1965-10-01 Impact factor: 14.307

PHYTOHEMAGGLUTININ ELICITATION OF SPECIFIC ANAMNESTIC IMMUNE RESPONSE IN VITRO.

Review 1. [Lymphocyte culture and its relation to clinical problems].

2. Immunoglobulin synthesis by human peripheral lymphocytes and thymocytes in vitro. Specificity of immunochemical methods and stimulation by phytohaemagglutinin.

3. Affinity of antigen for white cells and its relation to the induction of antibody formation.

Review 4. Symposium on in vitro studies of the immune response. II. Significance of the reaction of lymphoid cells to homologous tissue.

5. Secondary response in vitro to antigen phi x 174 phage.

6. Growth of Myxovirus parainfluenza type 3 in organ cultures of guinea pig tissue.

7. Hemopoietic spleen colony studies. IV. Phytohemagglutinin and hemopoietic regeneration.

8. The X-Y-Z scheme of immunocyte maturation. IV. The exhaustion of memory cells.

9. The effects of antigens and of phytohemagglutinin on rabbit spleen cell suspensions.

10. Studies on rabbit lymphocytes in vitro. 3. Proteins, RNA, and DNA synthesis by lymphocyte cultures after stimulation with phytohaemagglutinin, with staphylococcal filtrate, with antiallotype serum, and with heterologous antiserum to rabbit whole serum.