INITIATION OF MITOSIS IN RELATION TO THE CELL CYCLE FOLLOWING FEEDING OF STARVED CHICKENS.

Cellular proliferation of newly hatched chickens was depressed by starving them for 2.5 to 3.5 days. Starvation may hold proliferative cells in different parts of the cell cycle. In order to find where in the cell cycle these cells are held, the animals were fed and the following events were measured as a function of time after the start of feeding: (1) the mitotic index, and (2) the DNA synthetic index (number of cells in DNA synthesis 1 hour after injection of H(3)-thymidine). The duration of the cell's DNA synthetic period (S) was measured, permitting a more exact description of the cell cycle. Analysis of the duodenal and esophageal epithelia shows that feeding initiates cell division by stimulating cells from the G1 part of the mitotic cycle in the duodenum. In the esophagus some of the cells were either stopped or slowed down in G1, and another group of cells in G2. Feeding simultaneously stimulates both cell groups; the former moves into S, the latter into mitosis. The S period in starved animals is a little longer than that in normally fed animals but the extension can be attributed to a slightly decreased body temperature.