Literature DB >> 14149943

AN IMPROVED METHOD FOR THE SIMPLE AND ACCURATE COLORIMETRIC DETERMINATION OF UREA WITH EHRLICH'S REAGENT.

H YATZIDIS, M GARIDI, C VASSILIKOS, D MAYOPOULOU, A AKILAS.   

Abstract

An improved method for the colorimetric determination of urea is described. The method is simple, requiring a deproteinized filtrate obtained after treatment of a small quantity of whole oxalated blood, serum, plasma or urine with 12.5% trichloracetic acid containing 50 g. activated carbon. The addition of Ehrlich's reagent to this filtrate results in the immediate production of a stable yellow colour. The determination is accurate, very sensitive and rapid, taking place in only 10 minutes.

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Keywords:  BLOOD CHEMICAL ANALYSIS; COLORIMETRY; INDICATORS AND REAGENTS; UREA; URINE

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Year:  1964        PMID: 14149943      PMCID: PMC480710          DOI: 10.1136/jcp.17.2.163

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN: 0021-9746            Impact factor:   3.411


  1 in total

1.  A rapid method for the determination of urea in blood and urine.

Authors:  J M LEVINE; R LEON; F STEIGMANN
Journal:  Clin Chem       Date:  1961-10       Impact factor: 8.327

  1 in total

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