Literature DB >> 14090747

THE BASE COMPOSITION OF RIBONUCLEIC ACID IN LAMPBRUSH CHROMOSOMES, NUCLEOLI, NUCLEAR SAP, AND CYTOPLASM OF TRITURUS OOCYTES.

J E EDSTROEM, J G GALL.   

Abstract

The base composition of RNA's extracted from chromosomes, nucleoli, nuclear sap, and cytoplasm of Triturus oocytes has been determined by microelectrophoresis. The chromosomal RNA has a content of guanine+cytosine equal to that of DNA, but there is no complementarity in the composition as for DNA. Nuclear sap contains a highly variable RNA with a tendency towards high uracil values. Nucleolar and cytoplasmic RNA's are similar in composition and both are of the guanine-cytosine rich type. The chromosomes and nucleoli contain roughly equivalent amounts of RNA, somewhat less than is present in the nuclear sap. The RNA/DNA ratio of the whole chromosomes is about 10. However, the ratio in the synthetically active regions, the loops, is much higher, since the loops contain all the chromosomal RNA but only a small fraction of the DNA.

Entities:  

Keywords:  ADENINE; CELL NUCLEUS; CHROMOSOMES; CYTOSINE; ELECTROPHORESIS; EXPERIMENTAL LAB STUDY; GERM CELLS; GUANINE; RNA; SALAMANDERS; URACIL

Mesh：

Substances：

Year:  1963        PMID： 14090747      PMCID： PMC2106876          DOI： 10.1083/jcb.19.2.279

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  10 in total

1.  Isolation of a nuclear RNA fraction resembling DNA in its base composition.

Authors:  A SIBATANI; S R DE KLOET; V G ALLFREY; A E MIRSKY
Journal:  Proc Natl Acad Sci U S A       Date:  1962-03-15       Impact factor: 11.205

2.  Evidence for the complete DNA-dependence of RNA synthesis in isolated thymus nuclei.

Authors:  V G ALLFREY; A E MIRSKY
Journal:  Proc Natl Acad Sci U S A       Date:  1962-09-15       Impact factor: 11.205

3.  The isolation of DNA-like RNA and ribosomal RNA from the nucleolo-chromosomal apparatus of mammalian cells.

Authors:  G P GEORGIEV; V L MANTIEVA
Journal:  Biochim Biophys Acta       Date:  1962-07-09

4.  H3 uridine incorporation in lampbrush chromosomes.

Authors:  J G GALL; H G CALLAN
Journal:  Proc Natl Acad Sci U S A       Date:  1962-04-15       Impact factor: 11.205

5.  Reversal of mutant phenotypes by 5-fluorouracil: an approach to nucleotide sequences in messenger-RNA.

Authors:  S P CHAMPE; S BENZER
Journal:  Proc Natl Acad Sci U S A       Date:  1962-04-15       Impact factor: 11.205

6.  The role of deoxyribonucleic acid in ribonucleic acid synthesis. III. The inhibition of the enzymatic synthesis of ribonucleic acid and deoxyribonucleic acid by actinomycin D and proflavin.

Authors:  J HURWITZ; J J FURTH; M MALAMY; M ALEXANDER
Journal:  Proc Natl Acad Sci U S A       Date:  1962-07-15       Impact factor: 11.205

7.  Chromosomal synthesis of ribonucleic acid as shown by incorporation of uridine labelled with tritium.

Authors:  G PELLING
Journal:  Nature       Date:  1959-08-22       Impact factor: 49.962

8.  The site of ribonucleic acid synthesis in the isolated nucleus.

Authors:  J H RHO; J BONNER
Journal:  Proc Natl Acad Sci U S A       Date:  1961-10-15       Impact factor: 11.205

9.  Biological validity of amino acid codes deduced with synthetic ribonucleotide polymers.

Authors:  F LANNI
Journal:  Proc Natl Acad Sci U S A       Date:  1962-09-15       Impact factor: 11.205

10.  The intracellular distribution and heterogeneity of ribonucleic acid in starfish oocytes.

Authors:  J E EDSTROM; W GRAMPP; N SCHOR
Journal:  J Biophys Biochem Cytol       Date:  1961-12
  10 in total
  11 in total

Review 1.  The plurifunctional nucleolus.

Authors:  T Pederson
Journal:  Nucleic Acids Res       Date:  1998-09-01       Impact factor: 16.971

2.  E.B. Wilson Award Lecture, 1996. Differential gene action.

Authors:  D D Brown
Journal:  Mol Biol Cell       Date:  1997-04       Impact factor: 4.138

Review 3.  Multiparameter microscopic analysis of nucleolar structure and ribosomal gene transcription.

Authors:  M F Trendelenburg; O V Zatsepina; T Waschek; W Schlegel; H Tröster; D Rudolph; G Schmahl; H Spring
Journal:  Histochem Cell Biol       Date:  1996-08       Impact factor: 4.304

4.  Nucleoli and chromosomes: their relationships during the meiotic prophase of the human fetal oocyte.

Authors:  A Stahl; J M Luciani
Journal:  Humangenetik       Date:  1972

5.  Feulgen slope determinations of urodele nuclear DNA amounts.

Authors:  K Bachmann
Journal:  Histochemie       Date:  1970

6.  Base composition and metabolism of various RNA fractions in Acetabularia mediterranea.

Authors:  E Baltus; J E Edström; J Hanocq-Quertier; R Tencer; J Brachet
Journal:  Proc Natl Acad Sci U S A       Date:  1968-02       Impact factor: 11.205

7.  Cajal bodies, nucleoli, and speckles in the Xenopus oocyte nucleus have a low-density, sponge-like structure.

Authors:  Korie E Handwerger; Jason A Cordero; Joseph G Gall
Journal:  Mol Biol Cell       Date:  2004-10-27       Impact factor: 4.138

8.  Observations on the nucleolar and total cell body nucleic acid of injured nerve cells.

Authors:  W E Watson
Journal:  J Physiol       Date:  1968-06       Impact factor: 5.182

9.  Ultrastructure of the nuclear envelope of amphibian oocytes. IV. Chemical nature of the nuclear pore complex material.

Authors:  U Scheer
Journal:  Z Zellforsch Mikrosk Anat       Date:  1972

10.  Spheroidal and ring nucleoli in amphibian oocytes. Patterns of uridine incorporation and fine structural features.

Authors:  N J Lane
Journal:  J Cell Biol       Date:  1967-11       Impact factor: 10.539
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