Literature DB >> 1408665

A perfused-cuvette method for fluorimetric studies of non-adherent cells.

L C Schlichter1, P S Pennefather, C J van Staden, E A Valentine.   

Abstract

We have developed a system for immobilizing non-adherent cells for use in macroscopic fluorescence measurements in a perfused cuvette. Using normal human T lymphocytes loaded with the fluorescent Ca(2+)-indicator, fluo-3, we have validated the properties of these cells immobilized on clear, non-fluorescent fluorohalocarbon film (Aclar) using the non-charged cell adhesive, Cell-Tak.

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Year:  1992        PMID： 1408665     DOI： 10.1007/bf00374232

Source DB:  PubMed          Journal:  Pflugers Arch        ISSN： 0031-6768            Impact factor:   3.657


  8 in total

1.  Calculation of the concentrations of free cations and cation-ligand complexes in solutions containing multiple divalent cations and ligands.

Authors:  D A Goldstein
Journal:  Biophys J       Date:  1979-05       Impact factor: 4.033

2.  Mitogen-induced oscillations of cytosolic Ca2+ and transmembrane Ca2+ current in human leukemic T cells.

Authors:  R S Lewis; M D Cahalan
Journal:  Cell Regul       Date:  1989-11

Review 3.  Calcium and T lymphocyte activation.

Authors:  P Gardner
Journal:  Cell       Date:  1989-10-06       Impact factor: 41.582

4.  Calcium dependency of antigen-specific (T3-Ti) and alternative (T11) pathways of human T-cell activation.

Authors:  M J Weiss; J F Daley; J C Hodgdon; E L Reinherz
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

5.  Cl- channels in intact human T lymphocytes.

Authors:  P A Pahapill; L C Schlichter
Journal:  J Membr Biol       Date:  1992-01       Impact factor: 1.843

6.  Photochemically generated cytosolic calcium pulses and their detection by fluo-3.

Authors:  J P Kao; A T Harootunian; R Y Tsien
Journal:  J Biol Chem       Date:  1989-05-15       Impact factor: 5.157

7.  The relationship between mitogen-induced membrane potential changes and intracellular free calcium in human T-lymphocytes.

Authors:  P E Tatham; K O'Flynn; D C Linch
Journal:  Biochim Biophys Acta       Date:  1986-04-14

8.  Transmembrane signalling by the T cell antigen receptor. Perturbation of the T3-antigen receptor complex generates inositol phosphates and releases calcium ions from intracellular stores.

Authors:  J B Imboden; J D Stobo
Journal:  J Exp Med       Date:  1985-03-01       Impact factor: 14.307
  8 in total
  1 in total

1.  Criteria for perforated-patch recordings: ion currents versus dye permeation in human T lymphocytes.

Authors:  I Chung; L C Schlichter
Journal:  Pflugers Arch       Date:  1993-09       Impact factor: 3.657
  1 in total

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