| Literature DB >> 14056704 |
Abstract
Sea urchin embryos at the 64-cell stage were dissociated by treatment with trypsin and separated by centrifugation on a sucrose gradient. The large blastomeres have two and the small blastomeres have three bands of L-malate dehydrogenase activity, which are separated by disk microelectrophoresis on polyacrylamide gel, whereas unfertilized eggs have five.Entities:
Keywords: ECHINODERMATA; EMBRYO; EXPERIMENTAL LAB STUDY; MALATE DEHYDROGENASE
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Year: 1963 PMID: 14056704 DOI: 10.1126/science.142.3590.389
Source DB: PubMed Journal: Science ISSN: 0036-8075 Impact factor: 47.728