Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 EFFECTS OF MALIGNANT EFFUSIONS ON THE MITOTIC INDEX OF L STRAIN MOUSE CELLS GROWN IN TISSUE CULTURE.

Literature DB >> 14052976

EFFECTS OF MALIGNANT EFFUSIONS ON THE MITOTIC INDEX OF L STRAIN MOUSE CELLS GROWN IN TISSUE CULTURE.

Abstract

By employing a clone of L strain mouse fibroblasts (L(E)) which does not exhibit cell clumping and lysis (cytolytic antibody reaction), it was possible to screen for the presence of growth-regulating factors in human sera and effusions, exclusive of an antigen-antibody reaction. Under conditions of the test a mitotic index greater than 20% indicated the presence of a growth-promoting factor.A total of 11 pleural effusions was tested. Four of the eight malignant effusions possessed a growth-promoting factor, while none of the three non-malignant effusions or the one sample of human umbilical cord serum possessed such a factor. Overnight storage of the unfiltered effusions at 5 degrees C. resulted in complete loss of the growthpromoting activity.

Entities: Disease Species

Keywords: CELL DIVISION; COLCHICINE; CYTODIAGNOSIS; EXPERIMENTAL LAB STUDY; EXUDATES AND TRANSUDATES; MICE; NEOPLASM DIAGNOSIS; NEOPLASM IMMUNOLOGY; PLEURAL EFFUSION; TISSUE CULTURE

Mesh：

Substances：
Colchicine

Year: 1963 PMID： 14052976 PMCID： PMC1921851

Source DB: PubMed Journal: Can Med Assoc J ISSN： 0008-4409 Impact factor: 8.262

11 in total

9. Effect of human blood serum on tissue cultures. II. Development of resistance to toxic human serum in fibroblast-like cells (Earle's strain L) obtained from a C3H mouse.

Authors: S FEDOROFF; B COOK
Journal: J Exp Med Date: 1959-06-01 Impact factor: 14.307

10. Incorporation of 32P and adenine 14C into DNA by human bone marrow cells in vitro.

Authors: L G LAJTHA; R OLIVER; F ELLIS
Journal: Br J Cancer Date: 1954-06 Impact factor: 7.640

1 in total

1. Further studies on the effect of human effusions on the mitotic index of mammalian cells grown in cell culture.

Authors: S B Hrushovetz; R J Mulder
Journal: Can Med Assoc J Date: 1966-11-12 Impact factor: 8.262

1 in total

EFFECTS OF MALIGNANT EFFUSIONS ON THE MITOTIC INDEX OF L STRAIN MOUSE CELLS GROWN IN TISSUE CULTURE.

1. Cytotoxicity of human malignant effusions.

2. DNA synthesis in individual L-strain mouse cells.

3. Comparison of effect of cerebrospinal fluid and of blood serum on tissue cultures.

4. An improved tissue culture assay. III. Alternate methods for measuring cell growth.

5. Further studies on the growth-controlling action of human serum on HeLa cells.

6. Growth promoting agents in cell fractions of chick embryos.

7. Cancer antigens: how specific should they be?

8. Mitosis-stimulating properties of embryonic nucleo-protein constituents in cell culture.

9. Effect of human blood serum on tissue cultures. II. Development of resistance to toxic human serum in fibroblast-like cells (Earle's strain L) obtained from a C3H mouse.

10. Incorporation of 32P and adenine 14C into DNA by human bone marrow cells in vitro.

1. Further studies on the effect of human effusions on the mitotic index of mammalian cells grown in cell culture.