Literature DB >> 1404266

Development and evaluation of an enzyme-linked immunosorbent assay for Plasmodium vivax-VK247 sporozoites.

R A Wirtz1, J Sattabongkot, T Hall, T R Burkot, R Rosenberg.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) for the Plasmodium vivax-VK247 (variant) circumsporozoite (CS) protein was developed and evaluated using sporozoites produced by feeding mosquitoes on Thai patients with parasitologically confirmed P. vivax infections. The ELISA had a detection threshold of fewer than 50 sporozoites. Using this assay in conjunction with an ELISA for the VK210 polymorph, nearly 16% of the 235 P. vivax cases produced sporozoites positive only for the variant; 69% produced sporozoites positive only in the VK210 assay; and 15% were positive in both assays, indicating mixed infections. Twelve cases (5%) produced sporozoites negative in one assay and with unexpectedly low activity in the other ELISA, indicating the possibility of other CS protein polymorphs.

Entities:  

Mesh：

Year:  1992        PMID： 1404266     DOI： 10.1093/jmedent/29.5.854

Source DB:  PubMed          Journal:  J Med Entomol        ISSN： 0022-2585            Impact factor:   2.278


  33 in total

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