Literature DB >> 1401964

Optimization of cytotoxic assay by target cell retention of the fluorescent dye carboxyfluorescein diacetate (CFDA) and comparison with conventional 51CR release assay.

M Provinciali1, G Di Stefano, N Fabris.   

Abstract

This paper describes the comparison between a fluorimetric NK assay based on the target cell retention of fluorescent dye carboxyfluorescein diacetate (cFDA) and standard 51Cr release assay. The results provide several suggestions to improve the cytotoxic assay based on the use of the fluorogenic substrate showing that the measurements of cFDA retained by target cells represent a method of evaluating cytotoxicity completely comparable to the 51Cr release assay.

Entities:  

Mesh：

Substances：

Year:  1992        PMID： 1401964     DOI： 10.1016/0022-1759(92)90266-v

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  5 in total

1.  SITC cancer immunotherapy resource document: a compass in the land of biomarker discovery.

Authors:  Siwen Hu-Lieskovan; Srabani Bhaumik; Kavita Dhodapkar; Jean-Charles J B Grivel; Sumati Gupta; Brent A Hanks; Sylvia Janetzki; Thomas O Kleen; Yoshinobu Koguchi; Amanda W Lund; Cristina Maccalli; Yolanda D Mahnke; Ruslan D Novosiadly; Senthamil R Selvan; Tasha Sims; Yingdong Zhao; Holden T Maecker
Journal:  J Immunother Cancer       Date:  2020-12       Impact factor: 13.751

2.  Improving methods of assessing natural killer cell cytotoxicity.

Authors:  Sandra E Sephton; Helena C Kraemer; Eric Neri; Daniel P Stites; Inka Weissbecker; David Spiegel
Journal:  Int J Methods Psychiatr Res       Date:  2006       Impact factor: 4.035

3.  Relationship between 17-beta-estradiol and prolactin in the regulation of natural killer cell activity during progression of endometriosis.

Authors:  M Provinciali; G Di Stefano; M Muzzioli; G G Garzetti; A Ciavattini; N Fabris
Journal:  J Endocrinol Invest       Date:  1995-09       Impact factor: 4.256

4.  A fluorescence based cell adhesion assay using Terasaki plates.

Authors:  M R Novelli; W F Bodmer; H Durbin
Journal:  In Vitro Cell Dev Biol Anim       Date:  1995-02       Impact factor: 2.416

5.  Role of zinc and alpha2 macroglobulin on thymic endocrine activity and on peripheral immune efficiency (natural killer activity and interleukin 2) in cervical carcinoma.

Authors:  E Mocchegiani; A Ciavattini; L Santarelli; A Tibaldi; M Muzzioli; P Bonazzi; R Giacconi; N Fabris; G G Garzetti
Journal:  Br J Cancer       Date:  1999-01       Impact factor: 7.640
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.