| Literature DB >> 1401948 |
J F Murphy1, D H Davies, C J Smith.
Abstract
Monoclonal antibodies have been raised against adrenalin and noradrenalin and used as the basis of enzyme-linked immunosorbent assays (ELISA) to detect and estimate the concentrations of these catecholamines. Inhibition assays are described, with sensitive quantification in the range from 1 mg/ml to 100 pg/ml. Cross-reactivity assays reveal that neither assay is subject to interference by catecholamine metabolites at concentrations less than 100 ng/ml and 1 micrograms/ml respectively. Isolation and quantification of both catecholamines from clinical samples is discussed and the potential application of these ELISAs in a clinical setting is proposed.Entities:
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Year: 1992 PMID： 1401948 DOI： 10.1016/0022-1759(92)90216-g
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303