Literature DB >> 1400966

Purification, characterization, and seroactivity of a 20-kilodalton Brucella protein antigen.

M S Zygmunt1, F B Gilbert, G Dubray.   

Abstract

An internal protein was purified from cell extracts of Brucella melitensis B115 by a combination of preparative isoelectric focusing and high-performance size exclusion chromatography. The protein has an apparent molecular mass of 230 kDa as determined by size exclusion chromatography. The protein was resolved to a single band of 20 kDa after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The native protein had an isoelectric point of 4.9. The N-terminal sequence of the 20-kDa protein was determined. The 20-kDa protein has been identified as antigen A-2 with a previously described anti-antigen A-2 serum (B. Stemshorn, K. Nielsen, and B. Samagh, Can. J. Comp. Med. 45:77-81, 1981). Antigen A-2 reacted with sera from infected sheep in immunoblotting and may be useful in developing diagnostic tests for brucellosis.

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Year:  1992        PMID： 1400966      PMCID： PMC270495          DOI： 10.1128/jcm.30.10.2662-2667.1992

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  23 in total

1.  A protein isolated from Brucella abortus is a Cu-Zn superoxide dismutase.

Authors:  B L Beck; L B Tabatabai; J E Mayfield
Journal:  Biochemistry       Date:  1990-01-16       Impact factor: 3.162

2.  Copper staining: a five-minute protein stain for sodium dodecyl sulfate-polyacrylamide gels.

Authors:  C Lee; A Levin; D Branton
Journal:  Anal Biochem       Date:  1987-11-01       Impact factor: 3.365

3.  Enzyme-linked immunosorbent assay for differentiation of the antibody response of cattle naturally infected with Brucella abortus or vaccinated with strain 19.

Authors:  K Nielsen; J W Cherwonogrodzky; J R Duncan; D R Bundle
Journal:  Am J Vet Res       Date:  1989-01       Impact factor: 1.156

4.  Analysis of immune response: comparison of immunoblots after isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis using cytoplasmic protein extract from Brucella.

Authors:  M S Zygmunt; J C Martin; G Dubray
Journal:  FEMS Microbiol Lett       Date:  1990-08       Impact factor: 2.742

5.  Preparative separation of poliovirus structural polypeptides by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, copper staining and electroelution, and induction of monospecific antisera.

Authors:  U Tessmer; R Dernick
Journal:  Electrophoresis       Date:  1989-04       Impact factor: 3.535

6.  Cloning, expression, and occurrence of the Brucella Cu-Zn superoxide dismutase.

Authors:  B J Bricker; L B Tabatabai; B A Judge; B L Deyoe; J E Mayfield
Journal:  Infect Immun       Date:  1990-09       Impact factor: 3.441

7.  Antibody response to antigens distinct from smooth lipopolysaccharide complex in Brucella infection.

Authors:  G G Schurig; L M Jones; S L Speth; D T Berman
Journal:  Infect Immun       Date:  1978-09       Impact factor: 3.441

8.  Surface antigens of smooth brucellae.

Authors:  R Diaz; L M Jones; D Leong; J B Wilson
Journal:  J Bacteriol       Date:  1968-10       Impact factor: 3.490

9.  Vaccination against bovine brucellosis with a low dose of strain 19 administered by the conjunctival route. III. -- Serological response and immunity in the pregnant cow.

Authors:  M Plommet; R Fensterbank
Journal:  Ann Rech Vet       Date:  1976

10.  Separation of soluble Brucella antigens by gel-filtration chromatography.

Authors:  J R McGhee; B A Freeman
Journal:  Infect Immun       Date:  1970-07       Impact factor: 3.441
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  8 in total

1.  Characterization of a monoclonal antibody specific for Brucella smooth lipopolysaccharide and development of a competitive enzyme-linked immunosorbent assay to improve the serological diagnosis of brucellosis.

Authors:  V Weynants; D Gilson; A Cloeckaert; P A Denoel; A Tibor; P Thiange; J N Limet; J J Letesson
Journal:  Clin Diagn Lab Immunol       Date:  1996-05

2.  Humoral immune responses of Brucella-infected cattle, sheep, and goats to eight purified recombinant Brucella proteins in an indirect enzyme-linked immunosorbent assay.

Authors:  J J Letesson; A Tibor; G van Eynde; V Wansard; V Weynants; P Denoel; E Saman
Journal:  Clin Diagn Lab Immunol       Date:  1997-09

3.  Antibody and delayed-type hypersensitivity responses to Ochrobactrum anthropi cytosolic and outer membrane antigens in infections by smooth and rough Brucella spp.

Authors:  J Velasco; R Díaz; M J Grilló; M Barberán; C Marín; J M Blasco; I Moriyón
Journal:  Clin Diagn Lab Immunol       Date:  1997-05

4.  Enzyme-linked immunosorbent assay with partially purified cytosoluble 28-kilodalton protein for serological differentiation between Brucella melitensis-infected and B. melitensis Rev.1-vaccinated sheep.

Authors:  H Salih-Alj Debbarh; A Cloeckaert; G Bézard; G Dubray; M S Zygmunt
Journal:  Clin Diagn Lab Immunol       Date:  1996-05

5.  Specific bovine brucellosis diagnosis based on in vitro antigen-specific gamma interferon production.

Authors:  V Weynants; J Godfroid; B Limbourg; C Saegerman; J J Letesson
Journal:  J Clin Microbiol       Date:  1995-03       Impact factor: 5.948

6.  Characterization of an 18-kilodalton Brucella cytoplasmic protein which appears to be a serological marker of active infection of both human and bovine brucellosis.

Authors:  F A Goldbaum; J Leoni; J C Wallach; C A Fossati
Journal:  J Clin Microbiol       Date:  1993-08       Impact factor: 5.948

7.  Cloning and sequence analysis of a newly identified Brucella abortus gene and serological evaluation of the 17-kilodalton antigen that it encodes.

Authors:  F Hemmen; V Weynants; T Scarcez; J J Letesson; E Saman
Journal:  Clin Diagn Lab Immunol       Date:  1995-05

Review 8.  Molecular host-pathogen interaction in brucellosis: current understanding and future approaches to vaccine development for mice and humans.

Authors:  Jinkyung Ko; Gary A Splitter
Journal:  Clin Microbiol Rev       Date:  2003-01       Impact factor: 26.132
  8 in total

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