Literature DB >> 139928

A complete cleavage map of Neurospora crassa mtDNA obtained with endonucleases Eco RI and Bam HI.

P Terpstra, M Holtrop, A M Kroon.   

Abstract

A physical map of Neurospora crassa mitochondrial DNA has been constructed using specific fragments obtained with restriction endonucleases. The DNA has 5 cleavage sites for endonuclease Bam HI, 12 for endonuclease Eco RI and more than 30 for endonuclease Hind III. The sequence of the Eco RI and Bam HI fragments has been established by analysis of partial fragments. By digestion of the Eco RI fragments with Bam HI, a complete overlapping map has been constructed. The position of the largest Hind III fragment on this map has also been determined. The map is circular and the added molecular weight of the fragments is 40 - 10(6), which is in good agreement with earlier measurements on intact DNA, using the electron microscope.

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Year:  1977        PMID： 139928     DOI： 10.1016/0005-2787(77)90318-5

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  13 in total

1.  Transmission of mitochondrial deoxyribonucleic acid in Neurospora crassa sexual crosses.

Authors:  C A Mannella; T H Pittenger; A M Lambowitz
Journal:  J Bacteriol       Date:  1979-03       Impact factor: 3.490

2.  Laboratory of Physiological Chemistry, State University Groningen, Netherlands.

Authors:  H de Vries; J C de Jonge; H Bakker; H Meurs; A Kroon
Journal:  Nucleic Acids Res       Date:  1979       Impact factor: 16.971

3.  Restriction endonuclease cleavage map of mitochondrial DNA from Aspergillus nidulans.

Authors:  P P Stepień; U Bernard; H J Cooke; H Küntzel
Journal:  Nucleic Acids Res       Date:  1978-02       Impact factor: 16.971

4.  Molecular cloning and physical mapping of the Neurospora crassa 74-OR23-1A mitochondrial genome.

Authors:  J W Taylor; B D Smolich
Journal:  Curr Genet       Date:  1985       Impact factor: 3.886

5.  Mitochondrial DNA from Podospora anserina. I. Isolation and characterization.

Authors:  D J Cummings; L Belcour; C Grandchamp
Journal:  Mol Gen Genet       Date:  1979-03-27

6.  Unidirectional gene conversion associated with two insertions in neurospora crassa mitochondrial DNA.

Authors:  C A Mannella; A M Lambowitz
Journal:  Genetics       Date:  1979-11       Impact factor: 4.562

7.  Mitochondrial DNA from Podospora anserina. II. Properties of mutant DNA and multimeric circular DNA from senescent cultures.

Authors:  D J Cummings; L Belcour; C Grandchamp
Journal:  Mol Gen Genet       Date:  1979-03-27

8.  Structure and expression of the overlapping ND4L and ND5 genes of Neurospora crassa mitochondria.

Authors:  M A Nelson; G Macino
Journal:  Mol Gen Genet       Date:  1987-02

9.  Deletion mutants of Neurospora crassa mitochondrial DNA and their relationship to the "stop-start" growth phenotype.

Authors:  H Bertrand; R A Collins; L L Stohl; R R Goewert; A M Lambowitz
Journal:  Proc Natl Acad Sci U S A       Date:  1980-10       Impact factor: 11.205

10.  Characterization of a variant Neurospora crassa mitochondrial DNA which contains tandem reiterations of a 1.9 kb sequence.

Authors:  R A Collins; A M Lambowitz
Journal:  Curr Genet       Date:  1981-11       Impact factor: 3.886
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