[Acetylcholine in the bovine retina and the parasympathomimetic effect of pilocarpine].

Using titrations of acetylcholine (ACh) on guinea-pig ileum the authors have demonstrated that there is no significant statistical difference between the cholinergic activity of extracts of 150 mg of retinal tissue from pilocarpinised eyes (0.3 mug of ACh) and the activity of extracts from control eyes not subjected to pilocarpine (0.29 mug of ACh). The pilocarpine had been instilled into the bovine conjunctival sac at the rate of three drops of 3 p. 100 pilocarpine HCl ten times at intervals of three minutes. One hour after the last application the animals were killed and the eyes enucleated. The role of pilocarpine in the regulation of tissues in the posterior segment of the eye is discussed.