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Literature DB >> 13962188

The mitotic apparatus. Fine structure of the isolated unit.

Abstract

The fine structure of the mitotic apparatus isolated from the sea urchin egg has been investigated. The isolation was accomplished by lysis of metaphase eggs in a 1 M solution of hexanediol, buffered at pH 6. The fine structure of the isolated apparatus was studied after fixation with osmium tetroxide directly in the isolation medium. The spindle is composed of fine fibrils, approximately 20 mmicro in diameter, which appear tubular. Similar fibrils, radially oriented, are found in the aster. If the isolated mitotic apparatus is exposed to water at pH 6 before fixation, the structure is considerably modified. The most pronounced effects are an increase in the number of large membrane-bounded vesicles and in the amount of free granular material present. The conditions necessary for the fixation of the mitotic apparatus in dividing cells are discussed in the light of these observations on the isolated unit.

Entities: Chemical Species

Keywords: CELL DIVISION

Mesh：

Year: 1962 PMID： 13962188 PMCID： PMC2106157 DOI： 10.1083/jcb.15.2.279

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

14 in total

1. Nuclear and cytoplasmic differentiation in developing sperm of the crayfish, Cambaroides japonicus.

Authors: G YASUZUMI; G I KAYE; G D PAPPAS; H YAMAMOTO; I TSUBO
Journal: Z Zellforsch Mikrosk Anat Date: 1961

2. Improved fixative for astral rays and nuclear membrane of Tubifex embryos.

Authors: F E LEHMANN; V MANCUSO
Journal: Exp Cell Res Date: 1957-08 Impact factor: 3.905

3. The Isolation and Biochemical Characterization of the Mitotic Apparatus of Dividing Cells.

Authors: D Mazia; K Dan
Journal: Proc Natl Acad Sci U S A Date: 1952-09 Impact factor: 11.205

4. The electron microscopy of dividing cells.

Authors: G ROZSA; R W G WYCKOFF
Journal: Biochim Biophys Acta Date: 1950-11

5. Electron microscope studies on structure of mitotic figure.

Authors: H W BEAMS; T C EVANS; V VAN BREEMEN; W W BAKER
Journal: Proc Soc Exp Biol Med Date: 1950-08

6. Electron micrographs of the amphiaster in the whitefish blastula (Coregonus cluperformis).

Authors: H W BEAMS; T C EVANS; W W BAKER; V VAN BREEMEN
Journal: Anat Rec Date: 1950-07

7. The fine structure of the meiotic spindle of the crayfish.

Authors: A RUTHMANN
Journal: J Biophys Biochem Cytol Date: 1959-01-25

8. A device for staining tissue sections for electron microscopy.

Authors: L D PEACHEY
Journal: J Biophys Biochem Cytol Date: 1959-05-25

9. Electron microscope study of mitosis in sea urchin blastomeres.

Authors: P HARRIS
Journal: J Biophys Biochem Cytol Date: 1961-11

10. Staining of tissue sections for electron microscopy with heavy metals. II. Application of solutions containing lead and barium.

Authors: M L WATSON
Journal: J Biophys Biochem Cytol Date: 1958-11-25

15 in total

1. A functional mitotic spindle prepared from mammalian cells in culture.

Authors: W Z Cande; J Snyder; D Smith; K Summers; J R McIntosh
Journal: Proc Natl Acad Sci U S A Date: 1974-04 Impact factor: 11.205

2. Augmentation and dispersion of the in vivo mitotic apparatus of living marine eggs.

Authors: L I Rebhun; N Sawada
Journal: Protoplasma Date: 1969 Impact factor: 3.356

3. Characterization of the mitotic traction system, and evidence that birefringent spindle fibers neither produce nor transmit force for chromosome movement.

Authors: A Forer
Journal: Chromosoma Date: 1966 Impact factor: 4.316

The mitotic apparatus. Fine structure of the isolated unit.

1. Nuclear and cytoplasmic differentiation in developing sperm of the crayfish, Cambaroides japonicus.

2. Improved fixative for astral rays and nuclear membrane of Tubifex embryos.

3. The Isolation and Biochemical Characterization of the Mitotic Apparatus of Dividing Cells.

4. The electron microscopy of dividing cells.

5. Electron microscope studies on structure of mitotic figure.

6. Electron micrographs of the amphiaster in the whitefish blastula (Coregonus cluperformis).

7. The fine structure of the meiotic spindle of the crayfish.

8. A device for staining tissue sections for electron microscopy.

9. Electron microscope study of mitosis in sea urchin blastomeres.

10. Staining of tissue sections for electron microscopy with heavy metals. II. Application of solutions containing lead and barium.

1. A functional mitotic spindle prepared from mammalian cells in culture.

2. Augmentation and dispersion of the in vivo mitotic apparatus of living marine eggs.

3. Characterization of the mitotic traction system, and evidence that birefringent spindle fibers neither produce nor transmit force for chromosome movement.

4. The structural elements responsible for contraction in the ciliate Spirostomum.

5. Comparison of selected cryoprotective agents to stabilize meiotic spindles of human oocytes during cooling.

6. STRUCTURE OF THE MITOTIC SPINDLE IN L STRAIN FIBROBLASTS.

7. The mitotic apparatus. Structural changes after isolation.

8. ISOLATION OF SPINDLES FROM THE SURF CLAM SPISULA SOLIDISSIMA.

9. THE STRUCTURE AND FORMATION OF CILIA AND FILAMENTS IN RUMEN PROTOZOA.

10. THE ULTRASTRUCTURE OF MAUTHNER CELL SYNAPSES AND NODES IN GOLDFISH BRAINS.