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Literature DB >> 1394369

Choline acetyltransferase expression studied with an oligonucleotide probe.

Abstract

1. The localization of choline acetyltransferase messenger RNA has been studied using a digoxigenin-tailed complementary oligodeoxynucleotide probe for in situ hybridization. 2. Putative cholinergic cells of the rat and ferret spinal cord and the ferret retina were labeled. 3. This technique affords superior resolution compared to radioactively labeled probes, with apparently equal sensitivity.

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Year: 1992 PMID： 1394369 DOI： 10.1007/bf00734931

Source DB: PubMed Journal: Cell Mol Neurobiol ISSN： 0272-4340 Impact factor: 5.046

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References

16 in total

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Authors: R B Denman; D L Miller
Journal: Biotechniques Date: 1989-02 Impact factor: 1.993

2. Effects of central nervous system lesions on the expression of galanin: a comparative in situ hybridization and immunohistochemical study.

Authors: R Cortés; M J Villar; A Verhofstad; T Hökfelt
Journal: Proc Natl Acad Sci U S A Date: 1990-10 Impact factor: 11.205

3. Complementary DNAs for choline acetyltransferase from spinal cords of rat and mouse: nucleotide sequences, expression in mammalian cells, and in situ hybridization.

Authors: K Ishii; Y Oda; T Ichikawa; T Deguchi
Journal: Brain Res Mol Brain Res Date: 1990-02

4. A highly sensitive, nonradioactive DNA labeling and detection system.

Authors: R Martin; C Hoover; S Grimme; C Grogan; J Höltke; C Kessler
Journal: Biotechniques Date: 1990-12 Impact factor: 1.993

5. Complete sequence of a cDNA encoding an active rat choline acetyltransferase: a tool to investigate the plasticity of cholinergic phenotype expression.

Authors: A Brice; S Berrard; B Raynaud; S Ansieau; T Coppola; M J Weber; J Mallet
Journal: J Neurosci Res Date: 1989-07 Impact factor: 4.164

6. Localization of Drosophila neurons that contain choline acetyltransferase messenger RNA: an in situ hybridization study.

Authors: R P Barber; H Sugihara; M Lee; J E Vaughn; P M Salvaterra
Journal: J Comp Neurol Date: 1989-02-22 Impact factor: 3.215

Choline acetyltransferase expression studied with an oligonucleotide probe.

1. Use of photobiotinylated deoxyoligonucleotides to detect cloned DNA.

2. Effects of central nervous system lesions on the expression of galanin: a comparative in situ hybridization and immunohistochemical study.

3. Complementary DNAs for choline acetyltransferase from spinal cords of rat and mouse: nucleotide sequences, expression in mammalian cells, and in situ hybridization.

4. A highly sensitive, nonradioactive DNA labeling and detection system.

5. Complete sequence of a cDNA encoding an active rat choline acetyltransferase: a tool to investigate the plasticity of cholinergic phenotype expression.

6. Localization of Drosophila neurons that contain choline acetyltransferase messenger RNA: an in situ hybridization study.

7. Conservation of amino acid sequences between human and porcine choline acetyltransferase.

8. Specific detection of neuronal cell bodies: in situ hybridization with a biotin-labeled neurofilament cDNA probe.

Review 9. Recent developments in the use of synthetic oligonucleotides for in situ hybridization histochemistry.

10. Histological detection of messenger RNAs with biotinylated synthetic oligonucleotide probes.