Projections of anterior hypothalamic neurones to the dorsal and ventral periaqueductal grey in the rat.

Projections of neurones in the rostral hypothalamus to the periaqueductal grey matter (PAG) of the rat were investigated using retrograde tracing of red and green fluorescent latex microspheres. Microspheres were injected into one of 4 PAG sub-divisions, namely the dorsal, dorsolateral, ventral and ventrolateral parts. The patterns of retrogradely labelled neurones in the hypothalamus from each of the 4 PAG sub-divisions were found to differ and these are described. The precise nature of projections of neurones in the anterior hypothalamic area (AHA) was investigated and it was found that neurones within a circumscribed area of AHA, the lateral area of the anterior hypothalamus (LAAH), projected predominantly to the dorsolateral PAG while neurones in immediately adjacent areas projected to either dorsolateral or ventrolateral aspects of the PAG. Double retrograde tracing studies, where the two different colour beads were injected into different subdivisions of the PAG, gave rise to very few double labelled hypothalamic neurones, indicating that neurones in the hypothalamus project to only one sub-division of the PAG. The functional significance of these pathways is discussed in relation to mechanisms of autonomic and sensory control.