Serology of aerobic aquatic Actinomycetes. I. Factors involved in antiserum production and in vitro antigen-antibody reactions.

Guthrie, R. K. (North Texas State University, Denton), A. W. Roach, and J. K. Ferguson. Serology of aerobic aquatic actinomycetes. I. Factors involved in antiserum production and in vitro antigen-antibody reactions. J. Bacteriol. 84:313-317. 1962.-The use of adjuvants permits shortening of immunization schedules, which results in improved specificity of in vitro reactions using sonically disrupted actinomycete antigenic preparations. The demonstration of specific serological reactions and of cross reactions is simplified, specificity is increased, and multiplicity of reactions is detectable by the use of agar-gel diffusion techniques in the study of individual isolates.