Literature DB >> 1389162

Efficient purification of PCR products using ultrafiltration.

A M Krowczynska1, M B Henderson.   

Abstract

The use of disposable ultrafiltration devices for the purification of PCR-amplified DNA products from the other components of the reaction mixture is outlined. The advantages of using a 100,000 molecular weight cutoff membrane are discussed. The integrity of the membrane-purified DNA is checked by its successful use in sequencing, ligation and cloning procedures.

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Year:  1992        PMID: 1389162

Source DB:  PubMed          Journal:  Biotechniques        ISSN: 0736-6205            Impact factor:   1.993


  3 in total

1.  Thermal cycle dideoxy DNA sequencing.

Authors:  B E Slatko
Journal:  Mol Biotechnol       Date:  1996-12       Impact factor: 2.695

2.  Rapid diagnosis of human brucellosis by peripheral-blood PCR assay.

Authors:  M I Queipo-Ortuño; P Morata; P Ocón; P Manchado; J D Colmenero
Journal:  J Clin Microbiol       Date:  1997-11       Impact factor: 5.948

3.  Universal emergence of precore mutant hepatitis B virus along with seroconversion to anti-HBe irrespective of subsequent activity of chronic hepatitis B.

Authors:  K C Koh; H S Lee; C Y Kim
Journal:  Korean J Intern Med       Date:  1994-07       Impact factor: 2.884

  3 in total

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