Binding of human alpha-interferon in the brain tissue membranes of rat.

The receptor binding characteristics of human lymphoblastoid alpha-interferon (IFN) to human Daudi cell line was compared with its binding to membrane homogenates derived from various regions of rat brain. Rat brain membranes bound specifically a detectable amount of I-125 alpha interferon revealing the receptor affinity constant (Kd) of 3.9-4.2 x 10(-10) M and the Bmax was 0.9-1.7 fmoles/mg wet weight tissue depending on the analyzed brain region (the highest Bmax was obtained in the membranes from the hypothalamus region, the lowest one from the spinal cord membranes).