Actions of a metabotropic glutamate receptor agonist in immature and adult rat cerebellum.

The electrophysiological actions of the metabotropic glutamate receptor agonist 1S,3R-1-aminocyclopentane-1,3-dicarboxylic acid (ACPD) on Purkinje and granule cells were studied in immature and adult cerebellar slices. ACPD elicited a depolarising response when applied to Purkinje cells (EC50 approximately 20 microM). Granule cells hyperpolarised when exposed to low (3-10 microM) concentrations of ACPD; higher concentrations produced a depolarisation (EC50 approximately 40 microM) that was rapidly curtailed by a hyperpolarisation. The hyperpolarisation was abolished when Ca2+ was removed. In Purkinje cells, the amplitude of the depolarisation was greater in adult slices compared to those in immature slices. The responses were not blocked by ionotropic glutamate receptor antagonists or (L)-2-amino-3-phosphonopropionate (AP3).