Literature DB >> 1384961

Intracellular localization of human DNA repair enzyme methylguanine-DNA methyltransferase by antibodies and its importance.

T C Ayi1, K C Loh, R B Ali, B F Li.   

Abstract

The human DNA repair enzyme, methylguanine-DNA methyltransferase (MGMT, M(r) 21,000), which protects cells against the mutagenic effect of alkylating carcinogens, was found to be localized in the cell nucleus (except the nucleolus) by immunofluorescence staining using polyclonal and monoclonal antibodies. The supporting experiments came from differential staining of the MGMT-deficient (mer-) and -proficient (mer+) cells, Western blotting analysis, and specific antibody depletion studies with the immobilized fusion protein, GSTMGMT-glutathione-Sepharose. Its localization in the nucleus agrees with its biological function and possibly explains the ineffective protection of mammalian cells (mer-) transfected with the Escherichia coli MGMT genes from bifunctional alkylating agents.

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Year:  1992        PMID： 1384961

Source DB:  PubMed          Journal:  Cancer Res        ISSN： 0008-5472            Impact factor:   12.701


  8 in total

1.  Impact of rs12917 MGMT Polymorphism on [18F]FDG-PET Response in Pediatric Hodgkin Lymphoma (PHL).

Authors:  Stefanie Kewitz-Hempel; Lars Kurch; Michaela Cepelova; Ines Volkmer; Axel Sauerbrey; Elke Conrad; Stephanie Knirsch; Gabriele Pöpperl; Daniel Steinbach; Ambros J Beer; Christof M Kramm; Carsten-Oliver Sahlmann; Bernhard Erdlenbruch; Wolf-Dieter Reinbold; Andreas Odparlik; Osama Sabri; Regine Kluge; Martin S Staege
Journal:  Mol Imaging Biol       Date:  2019-12       Impact factor: 3.488

2.  Implication of localization of human DNA repair enzyme O6-methylguanine-DNA methyltransferase at active transcription sites in transcription-repair coupling of the mutagenic O6-methylguanine lesion.

Authors:  R B Ali; A K Teo; H K Oh; L S Chuang; T C Ayi; B F Li
Journal:  Mol Cell Biol       Date:  1998-03       Impact factor: 4.272

3.  The nuclear targeting and nuclear retention properties of a human DNA repair protein O6-methylguanine-DNA methyltransferase are both required for its nuclear localization: the possible implications.

Authors:  A Lim; B F Li
Journal:  EMBO J       Date:  1996-08-01       Impact factor: 11.598

4.  Specificities of human, rat and E. coli O6-methylguanine-DNA methyltransferases towards the repair of O6-methyl and O6-ethylguanine in DNA.

Authors:  L K Liem; A Lim; B F Li
Journal:  Nucleic Acids Res       Date:  1994-05-11       Impact factor: 16.971

5.  Expression of O-Alkylguanine-DNA Alkyltransferase in Normal and Malignant Bladder Tissue of Egyptian Patients.

Authors:  Abir A Saad; Heba Sh Kassem; Andrew C Povey; Geoffrey P Margison
Journal:  J Nucleic Acids       Date:  2010-10-17

Review 6.  Inhibiting the DNA damage response as a therapeutic manoeuvre in cancer.

Authors:  N J Curtin
Journal:  Br J Pharmacol       Date:  2013-08       Impact factor: 8.739

Review 7.  DNA-repair methyltransferase as a molecular device for preventing mutation and cancer.

Authors:  M Sekiguchi; Y Nakabeppu; K Sakumi; T Tuzuki
Journal:  J Cancer Res Clin Oncol       Date:  1996       Impact factor: 4.553

8.  Inhibition of DNA repair with MGMT pseudosubstrates: phase I study of lomeguatrib in combination with dacarbazine in patients with advanced melanoma and other solid tumours.

Authors:  H A Tawbi; L Villaruz; A Tarhini; S Moschos; M Sulecki; F Viverette; J Shipe-Spotloe; R Radkowski; J M Kirkwood
Journal:  Br J Cancer       Date:  2011-08-02       Impact factor: 7.640
  8 in total

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